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Titolo:
Quantification of Epstein-Barr virus load in peripheral blood of human immunodeficiency virus-infected patients using real-time PCR
Autore:
Dehee, A; Asselot, C; Piolot, T; Jacomet, C; Rozenbaum, W; Vidaud, M; Garbarg-Chenon, A; Nicolas, JC;
Indirizzi:
Hop Trousseau, Serv Virol, EA 2391, F-75012 Paris, France Hop Trousseau Paris France F-75012 Virol, EA 2391, F-75012 Paris, France Hop Rothschild, Serv Virol, EA 2391, F-75571 Paris, France Hop RothschildParis France F-75571 irol, EA 2391, F-75571 Paris, France Hop Rothschild, Serv Malad Infect, EA 2391, F-75571 Paris, France Hop Rothschild Paris France F-75571 fect, EA 2391, F-75571 Paris, France Fac Sci Pharmaceut & Biol Paris 5, Genet Mol Lab, Paris, France Fac Sci Pharmaceut & Biol Paris 5 Paris France t Mol Lab, Paris, France
Titolo Testata:
JOURNAL OF MEDICAL VIROLOGY
fascicolo: 3, volume: 65, anno: 2001,
pagine: 543 - 552
SICI:
0146-6615(200111)65:3<543:QOEVLI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; POSTTRANSPLANT LYMPHOPROLIFERATIVE DISEASE; QUANTITATIVE COMPETITIVE PCR; ORGAN TRANSPLANT RECIPIENTS; LONG TERMINAL REPEAT; CYTOTOXIC T-CELLS; B-CELLS; IN-VIVO; EBV PERSISTENCE; HOMOSEXUAL MEN;
Keywords:
EBV; real-time quantitative; PCR; TaqMan; HIV;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Dehee, A Hop Trousseau, Microbiol Serv, 26 Rue Dr A Netter, F-75012 Paris,France Hop Trousseau 26 Rue Dr A Netter Paris France F-75012 is, France
Citazione:
A. Dehee et al., "Quantification of Epstein-Barr virus load in peripheral blood of human immunodeficiency virus-infected patients using real-time PCR", J MED VIROL, 65(3), 2001, pp. 543-552

Abstract

Epstein-Barr virus (EBV) reactivation is more likely to occur in immunocompromised patients with subsequent higher susceptibility to EBV-associated lymphoproliferations. In contrast to transplant recipients, limited data areavailable concerning the EBV load in HIV-infected patients, with or without AIDS-related non-Hodgkin's lymphomas. We developed a TaqMan real-time PCRassay, allowing both the EBV genome and a cellular gene to be quantified in order to obtain a reliable normalized measurement of the EBV load in peripheral blood mononuclear cells (PBMCs). With a wide 6-log(10) quantification range and inter-assay variations of less than 24%, this quantitative PCR was sufficiently accurate and reproducible for routine follow-up. The EBV load was determined in PBMCs from 113 HIV-infected patients, 11 patients with primary HIV infection and 24 HIV-seronegative healthy controls. The ratesof EBV detection were similar in the three groups. However, EBV loads werehigher in the HIV-infected group (P <0.00001) except for the patients withprimary HIV infection. Unexpectedly, EBV loads were not correlated with the clinical stages of HIV infection or HIV replication, and did not depend on the degree of immunodepression, as judged by CD4(+) counts. This study contributes towards the definition of the baseline EBV load during HIV infection and stresses the broad inter-individual variability of the EBV load in HIV-infected patients. Real-time PCR provides a useful tool that can be used in further longitudinal studies to assess the relevance of the EBV load to identify HIV-infected patients with a high risk of EBV-associated lymphoproliferations. (C) 2001 Wiley- Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 07:12:57