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Titolo:
Factors affecting Agrobacterium-mediated gene transfer and the selection of transgenic calli in paper shell almond (Prunus dulcis Mill.)
Autore:
Ainsley, PJ; Collins, GG; Sedgley, M;
Indirizzi:
Univ Adelaide, Plant Res Ctr, Dept Hort Viticulture & Oenol, Glen Osmond, SA 5064, Australia Univ Adelaide Glen Osmond SA Australia 5064 en Osmond, SA 5064, Australia
Titolo Testata:
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
fascicolo: 5, volume: 76, anno: 2001,
pagine: 522 - 528
SICI:
1462-0316(200109)76:5<522:FAAGTA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
COAT PROTEIN GENE; PLUM POX VIRUS; SHOOT REGENERATION; LEAF EXPLANTS; TRANSFORMATION; PLANTS; APPLE; ACETOSYRINGONE; TUMEFACIENS; INVITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Ainsley, PJ Univ Adelaide, Plant Res Ctr, Dept Hort Viticulture & Oenol, PMB1,Waite Campus, Glen Osmond, SA 5064, Australia Univ Adelaide PMB1,Waite Campus Glen Osmond SA Australia 5064
Citazione:
P.J. Ainsley et al., "Factors affecting Agrobacterium-mediated gene transfer and the selection of transgenic calli in paper shell almond (Prunus dulcis Mill.)", J HORT SCI, 76(5), 2001, pp. 522-528

Abstract

Factors influencing the transfer of an intron-containing P-glucuronidase gene (GUS) to micropropagated leaf explants of the paper shell almond (Prunus dulcis Mill.) cultivars Nonpareil and Ne Plus Ultra were studied during the early steps of an Agrobacterium tumefaciens-mediated transformation procedure. The gene transfer process was evaluated by counting the number of GUS-positive leaf zones 4 d after cocultivation, as well as by counting numbers of GUS-positive calli developing on explants six weeks post-cocultivation. Of the two A. tumefaciens strains tested, EHA105 was more effective thanLBA4404, and cocultivation over a 4 d period was the optimum time for bothalmond cultivars. The presence of acetosyringone in the bacterial, plant preculture and cocultivation media had a varied affect on gene transfer efficiency, its influence being dependent on genotype and bacterial strain. Using an optimized procedure, 1500 leaf explants of both genotypes were transformed. While no transgenic plants were recovered, PCR and Southern blot analysis confirmed the presence of the transgene and its stable integration incallus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 14:51:49