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Titolo:
Response of human CD34(+) cells to CXC, CC, and CX3C chemokines: Implications for cell migration and activation
Autore:
Liesveld, JL; Rosell, K; Panoskaltsis, N; Belanger, T; Harbol, A; Abboud, CN;
Indirizzi:
Univ Rochester, Med Ctr, Hematol Oncol Unit, Dept Med, Rochester, NY 14642USA Univ Rochester Rochester NY USA 14642 t, Dept Med, Rochester, NY 14642USA
Titolo Testata:
JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH
fascicolo: 5, volume: 10, anno: 2001,
pagine: 643 - 655
SICI:
1525-8165(200110)10:5<643:ROHCCT>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC PROGENITOR CELLS; HUMAN BONE-MARROW; MACROPHAGE INFLAMMATORY PROTEIN-1-ALPHA; MICROVASCULAR ENDOTHELIAL-CELLS; TRANSENDOTHELIAL MIGRATION; ADHESION MOLECULES; NOD/SCID MICE; T-LYMPHOCYTES; IN-VITRO; PROLIFERATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Liesveld, JL Univ Rochester, Med Ctr, Hematol Oncol Unit, Dept Med, Box 610,601 ElmwoodAve, Rochester, NY 14642 USA Univ Rochester Box 610,601 Elmwood Ave Rochester NY USA 14642
Citazione:
J.L. Liesveld et al., "Response of human CD34(+) cells to CXC, CC, and CX3C chemokines: Implications for cell migration and activation", J HEMATH ST, 10(5), 2001, pp. 643-655

Abstract

Ultrastructural studies of marrow and examination of the in vivo processesof stem cell homing and mobilization show that multipotential hematopoietic progenitors are able to traverse endothelial cells. The regulation of this process by various classes of chemokines was studied in this report, using an in vitro model of transendothelial migration. Human umbilical vein endothelial cells (HU-VECs) or bone marrow-derived endothelial cells (BMECs) were grown to confluence on 3-mum microporous membrane inserts and placed in24-well culture plates. CD34(+) cells isolated from normal volunteer donormarrow by immunoadsorption or magnetic bead selection techniques were added to the inserts and various individual chemokines were added to the lower chamber of the culture plates in serum-free conditions. After 24 h, the percentage of transmigrated cells was determined. A mean of 8.5% of unfractionated marrow CD34(+) populations migrated, and all chemokines tested, with the exception of macrophage inflammatory protein-1 alpha (MIP-1 alpha), had some positive effect on this migration. The greatest effects were seen withstroma-derived factor-1 alpha (SDF-1 alpha) and stroma-derived factor-1 beta (SDF-1 beta), with lesser effects noted for other chemokines and cytokines. When the CD34(+) population was subselected for expression of CD38, a greater fraction of the CD38(-) cells migrated as compared to the CD38(+) fraction. CD34(+) cells isolated from mobilized peripheral blood and cord blood also migrated in response to chemokines. Chemokines of the CC, CXC, and CX3C classes as well as other hematopoietic cytokines may modulate the process of stem cell transmigration of endothelial cells. Further understandingof this process may help elucidate the mechanism of stem cell mobilizationand homing.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:16:29