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Titolo:
Ectopic expression of CCAAT/enhancer binding protein beta (C/EBP beta) in long-term bone marrow cultures induces granulopoiesis and alters stromal cell function
Autore:
Popernack, PM; Truong, LT; Kamphuis, M; Henderson, AJ;
Indirizzi:
Penn State Univ, Dept Vet Sci, Immunol Res Labs, University Pk, PA 16802 USA Penn State Univ University Pk PA USA 16802 s, University Pk, PA 16802 USA Penn State Univ, Grad Program Pathobiol, University Pk, PA 16802 USA Penn State Univ University Pk PA USA 16802 l, University Pk, PA 16802 USA
Titolo Testata:
JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH
fascicolo: 5, volume: 10, anno: 2001,
pagine: 631 - 642
SICI:
1525-8165(200110)10:5<631:EEOCBP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; HEMATOPOIETIC STEM-CELLS; MONOCYTE CHEMOATTRACTANT PROTEIN-1; NF-KAPPA-B; TRANSCRIPTION FACTORS; RETROVIRAL VECTOR; GENE-EXPRESSION; MAMMALIAN-CELLS; FAMILY MEMBERS; DEFICIENT MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Henderson, AJ Penn State Univ, Dept Vet Sci, Immunol Res Labs, 115 HenningBldg, University Pk, PA 16802 USA Penn State Univ 115 Henning Bldg University Pk PA USA 16802
Citazione:
P.M. Popernack et al., "Ectopic expression of CCAAT/enhancer binding protein beta (C/EBP beta) in long-term bone marrow cultures induces granulopoiesis and alters stromal cell function", J HEMATH ST, 10(5), 2001, pp. 631-642

Abstract

CCAAT/enhancer binding proteins (C/EBP) have been demonstrated to impact directly the development of multiple hematopoietic lineages. However, the role of C/EBP beta in the differentiation of various hematopoietic lineages has not been thoroughly examined. We used primary bone marrow cultures to assess directly the ability of C/EBP beta to influence myelopoiesis. Retroviral expression vectors were used to express C/EBP beta ectopically in murineprimary long-term bone marrow cultures. The differentiation potential of these cells was determined using hematopoietic colony assays and differential staining of cells within the cultures. Bone marrow cultures that overexpressed C/EBP beta had fewer myeloid progenitors and a significant increase in the number of granulocytes. The ability of C/EBP beta to alter hematopoiesis in vitro was dependent on the presence of the transcriptional activation domain because LIP, which lacks this functional domain, did not decrease the ability of bone marrow cultures to support myeloid progenitors. These data also show that C/EBP beta influences hematopoiesis by altering stromal cell function rather than the intrinsic developmental potential of myeloid progenitor cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 06:46:58