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Titolo:
HIV-1 infection of placental cord blood monocyte-derived dendritic cells
Autore:
Folcik, RM; Merrill, JD; Li, Y; Guo, CJ; Douglas, SD; Starr, SE; Ho, WZ;
Indirizzi:
Univ Penn, Childrens Hosp Philadelphia, Sch Med, Div Infect Dis & Immunol,Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 & Immunol,Philadelphia, PA 19104 USA Univ Penn, Childrens Hosp Philadelphia, Sch Med, Div Neonatol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Neonatol, Philadelphia, PA 19104 USA
Titolo Testata:
JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH
fascicolo: 5, volume: 10, anno: 2001,
pagine: 609 - 620
SICI:
1525-8165(200110)10:5<609:HIOPCB>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; T-CELLS; LANGERHANS CELLS; PERIPHERAL-BLOOD; PRODUCTIVE INFECTION; PROGENITOR CELLS; NAIVE PRECURSORS; GM-CSF; MACROPHAGES; ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Ho, WZ Univ Penn, Childrens Hosp Philadelphia, Sch Med, Div Infect Dis & Immunol,34th St & Civ Ctr Blvd, Philadelphia, PA 19104 USA Univ Penn 34th St& Civ Ctr Blvd Philadelphia PA USA 19104 9104 USA
Citazione:
R.M. Folcik et al., "HIV-1 infection of placental cord blood monocyte-derived dendritic cells", J HEMATH ST, 10(5), 2001, pp. 609-620

Abstract

Dendritic cells (DC), the most potent antigen-presenting cells (APC), havebeen implicated as the initial targets of HIV infection in skin and mucosal surfaces. DC can be generated in vitro from blood-isolated CD14(+) monocytes or CD34(+) hematopoietic progenitor cells in the presence of various cytokines. In this study, we investigated whether monocytes obtained from placental cord blood are capable of differentiation into dendritic cells when cultured with a combination of cytokines-granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-4 (IL-4), and tumor necrosis factor-alpha (TNF-alpha). We then examined HIV infection, HIV receptor (CD4, CCR5) expression, and beta -chemokine [macrophage inflammatory protein-1 alpha and-1 beta (MIP-1 alpha, MIP-1 beta)] production by placental cord monocyte-derived dendritic cells (MDDC) as compared to that of autologous cord monocyte-derived macrophages (MDM). Monocytes isolated from placental cord blood differentiate into DC after 7 days in culture with the mixture of cytokines, as demonstrated by development of characteristic DC morphology, loss of CD14 expression, and gain of CD83, a marker for mature DC. Mature cord MDDC had significantly lower susceptibility to M-tropic ADA (CCR5-dependent) envelope-pseudotyped HIV infection in comparison to autologous placental cord MDM, whereas there was no significant difference in virus replication in cord MDDC and MDM infected with murine leukemia virus envelope-pseudotyped HIV (HIV receptor-independent). This limited susceptibility of cord MDDC to M-tropic HIV infection may be due to lower expression of CD4 and CCR5 on thecell membrane and higher production of MIP-1 alpha and MIP-1 beta. These data provide important information toward our understanding of the biological properties of cord MDDC in relation to HIV infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 02:10:03