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Titolo:
Clathrin exchange during clathrin-mediated endocytosis
Autore:
Wu, XF; Zhao, XH; Baylor, L; Kaushal, S; Eisenberg, E; Greene, LE;
Indirizzi:
NHLBI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA NHLBI Bethesda MD USA 20892 I, Cell Biol Lab, NIH, Bethesda, MD 20892 USA
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 2, volume: 155, anno: 2001,
pagine: 291 - 300
SICI:
0021-9525(20011015)155:2<291:CEDCE>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
COATED VESICLE FORMATION; CONFORMATIONAL-CHANGES; PIT FORMATION; DYNAMIN; RECEPTOR; PROTEIN; CELLS; CHOLESTEROL; AUXILIN; BINDING;
Keywords:
clathrin; exchange; FRAP; endocytosis; Hsc70;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Greene, LE NHLBI, Cell Biol Lab, NIH, 50 South Dr,Rm 2537 MSC 8017, Bethesda, MD 20892 USA NHLBI 50 South Dr,Rm 2537 MSC 8017 Bethesda MD USA 20892 92 USA
Citazione:
X.F. Wu et al., "Clathrin exchange during clathrin-mediated endocytosis", J CELL BIOL, 155(2), 2001, pp. 291-300

Abstract

During clathrin-mediated endocytosis, clathrin-coated pits invaginate to form clathrin-coated vesicles (CVs). Since clathrin-coated pits are planar structures, whereas CVs are spherical, there must be a structural rearrangement of clathrin as invagination occurs. This could occur through simple addition of clathrin triskelions to the edges of growing clathrin-coated pits with very little exchange occurring between clathrin in the pits and free clathrin in the cytosol, or it could occur through large scale exchange of free and bound clathrin. In the present study, we investigated this questionby studying clathrin exchange both in vitro and in vivo. We found that in vitro clathrin in CVs and clathrin baskets do not exchange with free clathrin even in the presence of Hsc70 and ATP where partial uncoating occurs. However, surprisingly FRAP studies on clathrin-coated pits labeled with greenfluorescent protein-clathrin light chains in HeLa cells show that even when endocytosis is blocked by expression of a dynamin mutant or depletion of cholesterol from the membrane, replacement of photobleached clathrin in coated pits on the membrane occurs at almost the same rate and magnitude as when endocytosis is-occurring. Furthermore, very little of this replacement is due to dissolution of old pits and reformation of new ones; rather, it iscaused by a rapid ATP-dependent exchange of clathrin in the pits with freeclathrin in the cytosol. On the other hand, consistent with the in vitro data both potassium depletion and hypertonic sucrose, which have been reported to transform clathrin-coated pits into clathrin cages just below the surface of the plasma membrane, not only block endocytosis but also block exchange of clathrin. Taken together, these data show that ATP-dependent exchange of free and bound clathrin is a fundamental property of clathrin-coated pits, but not clathrin baskets, and may be involved in a structural rearrangement of clathrin as clathrin-coated pits invaginate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 21:51:43