Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Losartan inhibits the angiotensin II-induced modifications on fibrinolysisand matrix deposition by primary human vascular smooth muscle cells
Autore:
Papakonstantinou, E; Roth, M; Kokkas, B; Papadopoulos, C; Karakiulakis, G;
Indirizzi:
Aristotelian Univ Thessaloniki, Sch Med, Dept Pharmacol, GR-54006 Thessaloniki, Greece Aristotelian Univ Thessaloniki Thessaloniki Greece GR-54006 niki, Greece Aristotelian Univ Thessaloniki, Sch Med, Dept Cardiol, GR-54006 Thessaloniki, Greece Aristotelian Univ Thessaloniki Thessaloniki Greece GR-54006 niki, Greece Univ Basel Hosp, Dept Res, CH-4031 Basel, Switzerland Univ Basel Hosp Basel Switzerland CH-4031 es, CH-4031 Basel, Switzerland
Titolo Testata:
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
fascicolo: 5, volume: 38, anno: 2001,
pagine: 715 - 728
SICI:
0160-2446(200111)38:5<715:LITAIM>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR INHIBITOR-1; SPONTANEOUSLY HYPERTENSIVE RATS; STIMULATES COLLAGEN-SYNTHESIS; GROWTH-FACTOR-BETA; MESANGIAL CELLS; HYALURONIC-ACID; GENE-EXPRESSION; HEART-DISEASE; MESSENGER-RNA; IN-VIVO;
Keywords:
losartan; matrix metalloproteinase-2; TIMPs; tissue plasminogen activator; plasminogen activator inhibitor-1 glycosaminoglycans;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Karakiulakis, G Aristotelian Univ Thessaloniki, Sch Med, Dept Pharmacol, GR-54006 Thessaloniki, Greece Aristotelian Univ Thessaloniki Thessaloniki Greece GR-54006
Citazione:
E. Papakonstantinou et al., "Losartan inhibits the angiotensin II-induced modifications on fibrinolysisand matrix deposition by primary human vascular smooth muscle cells", J CARDIO PH, 38(5), 2001, pp. 715-728

Abstract

Disorders in the fibrinolytic and renin-angiotensin-aldosterone systems and excessive extracellular matrix (ECM) deposition are determinant factors in several pathologic manifestations of vascular and cardiac tissue. We usedprimary human vascular smooth muscle cells (VSMC) and studied the effects of losartan on angiotensin II (Ang II)-mediated (a) DNA synthesis, (b) secretion of tissue-type plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1). (c) secretion of matrix metalloprotease-2 (MMP-2) activity and tissue inhibitors of MMPs (TIMPs), and (d) synthesis of glycosaminoglycans. VSMC cultures, established from human pulmonary arteries, were treated with Ang II (0.1 nM-1 muM) and/or losartan (0.1-10 muM), for 24 or 48h. DNA synthesis was assessed by incorporation of H-3-thymidine into VSMC,secreted tPA, PAI-1, and TIMPs by enzyme-linked immunosorbent assay, MMP-2activity by gelatin zymography, and glycosaminoglycan synthesis by H-3-glucosamine incorporation. Ang II (1 muM) enhanced DNA synthesis and secretionof PAI-1 and glycosaminoglycans and decreased secretion of MMP-2 activity and tPA, whereas it had no effect on secretion of TIMPs and glycosaminoglycans associated with cell layers. The Ang II-mediated effects were reversed by losartan, in a concentration-dependent manner. Losartan alone increased secretion of tPA, MMP-2 activity, and TIMPs and decreased secretion of PAI-1. These results indicate that AT, receptors are implicated in Ang II-mediated disorders of fibrinolysis and excessive ECM deposition by VSMC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 17:12:00