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Titolo:
Sak serine-threonine kinase acts as an effector of Tec tyrosine kinase
Autore:
Yamashita, Y; Kajigaya, S; Yoshida, K; Ueno, S; Ota, J; Ohmine, K; Ueda, M; Miyazato, A; Ohya, K; Kitamura, T; Ozawa, K; Mano, H;
Indirizzi:
Jichi Med Sch, Div Funct Genom, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Div Cardiol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Div Hematol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan NHLBI, Hematol Branch, NIH, Bethesda, MD 20892 USA NHLBI Bethesda MD USA 20892 , Hematol Branch, NIH, Bethesda, MD 20892 USA Univ Tokyo, Inst Med Sci, Dept Hematopoiet Factors, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 Hematopoiet Factors, Tokyo 1088639, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 42, volume: 276, anno: 2001,
pagine: 39012 - 39020
SICI:
0021-9258(20011019)276:42<39012:SSKAAA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-SERINE/THREONINE KINASE; MOLECULAR-CLONING; DROSOPHILA; GENE; IDENTIFICATION; EXPRESSION; DEGRADATION; ENCODES; GROWTH; INVIVO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Mano, H Jichi Med Sch, Div Funct Genom, 3311-1 Yakushiji, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch 3311-1 Yakushiji Minami Kawachi Tochigi Japan 3290498
Citazione:
Y. Yamashita et al., "Sak serine-threonine kinase acts as an effector of Tec tyrosine kinase", J BIOL CHEM, 276(42), 2001, pp. 39012-39020

Abstract

The murine sak gene encodes a putative serine-threonine kinase which is homologous to the members of the Plk/Polo family. Although Sak protein is presumed to be involved in cell growth mechanism, efforts have failed to demonstrate its kinase activity. Little has been, therefore, elucidated how Sak is regulated and how Sak contributes to cell proliferation. Tee is a cytoplasmic protein-tyrosine kinase (PTK) which becomes activated by the stimulation of cytokine receptors, lymphocyte surface antigens, heterotrimeric G protein-linked receptors, and integrins. To clarify the in vivo function of Tee, we have tried to isolate the second messengers of Tee by using the yeast two-hybrid screening. One of such Tec-binding proteins turned out to be Sak. In human kidney 293 cells, Sak became tyrosine-phosphorylated by Tee, and the serine-threonine kinase activity of Sak was detected only under the presence of Tee, suggesting Sak to be an effector molecule of Tee. In addition, Tee activity efficiently protects Sak from the "PEST" sequence-dependent proteolysis. Internal deletion of the PEST sequences led to the stabilization of Sak proteins, and expression of these mutants acted suppressive tocell growth. Our data collectively supports a novel role of Sak acting in the PTK-mediated signaling pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 23:51:44