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Titolo:
A multiplex PCR-based method derived from random amplified polymorphic DNA(RAPD) markers for the identification of species of the Anopheles minimus group in Southeast Asia
Autore:
Kengne, P; Trung, HD; Baimai, V; Coosemans, M; Manguin, S;
Indirizzi:
IRD, Dept Soc & Sante, Montpellier, France IRD Montpellier FranceIRD, Dept Soc & Sante, Montpellier, France Natl Inst Malariol Parasitol & Entomol, Hanoi, Vietnam Natl Inst Malariol Parasitol & Entomol Hanoi Vietnam ol, Hanoi, Vietnam Mahidol Univ, Dept Biol, Bangkok, Thailand Mahidol Univ Bangkok Thailand ahidol Univ, Dept Biol, Bangkok, Thailand Inst Trop Med, Dept Parasitol, Antwerp, Belgium Inst Trop Med Antwerp Belgium rop Med, Dept Parasitol, Antwerp, Belgium IRD, Ctr Biol & Gest Populat, Montpellier, France IRD Montpellier France RD, Ctr Biol & Gest Populat, Montpellier, France
Titolo Testata:
INSECT MOLECULAR BIOLOGY
fascicolo: 5, volume: 10, anno: 2001,
pagine: 427 - 435
SICI:
0962-1075(200110)10:5<427:AMPMDF>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULICIDAE; DIPTERA; MALARIA; COMPLEX; THAILAND; MYZOMYIA; VILLAGE;
Keywords:
Anopheles minimus; hybrizymes; multiplex PCR; malaria vector; Southeast Asia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Manguin, S CBGP, Campus Int Baillarguet,CS 30016, F-34988 Montferrier Sur Lez, France CBGP Campus Int Baillarguet,CS 30016 Montferrier Sur Lez France F-34988
Citazione:
P. Kengne et al., "A multiplex PCR-based method derived from random amplified polymorphic DNA(RAPD) markers for the identification of species of the Anopheles minimus group in Southeast Asia", INSEC MOL B, 10(5), 2001, pp. 427-435

Abstract

Effective control of Anopheles minimus s.I., an important malaria vector in Southeast Asia, is based on the accurate identification of species withinAn. minimus complex, which cannot be distinguished using morphological characters. Derived from individual random amplified polymorphic DNA markers, sequence characterized amplified regions were analysed for the design of species-specific paired-primers. Combination of these primers resulted in thedevelopment of a simple, robust multiplex PCR able to identify both species An. minimus A and C belonging to the complex, hybrids AC, and three sympatric and closely related species, An. aconitus, An. pampanai and An. varuna. Hybrids AC do not possess alleles of both parents but exhibit novel adaptive potentials resulting from recombination among parental genes leading tohybrizyme.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:52:22