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Titolo:
Potential role for a carbohydrate moiety in anti-Candida activity of humanoral epithelial cells
Autore:
Steele, C; Leigh, J; Swoboda, R; Ozenci, H; Fidel, PL;
Indirizzi:
Louisiana State Univ, Hlth Sci Ctr, Dept Microbiol Immunol & Parasitol, New Orleans, LA 70112 USA Louisiana State Univ New Orleans LA USA 70112 , New Orleans, LA 70112 USA Louisiana State Univ, Sch Dent, Dept Gen Dent, New Orleans, LA 70119 USA Louisiana State Univ New Orleans LA USA 70119 , New Orleans, LA 70119 USA
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 11, volume: 69, anno: 2001,
pagine: 7091 - 7099
SICI:
0019-9567(200111)69:11<7091:PRFACM>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPERIMENTAL VAGINAL CANDIDIASIS; T-CELLS; ALBICANS; RESPONSES; IMMUNITY; GROWTH; CALPROTECTIN; ANTIBIOTICS; RESISTANCE; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Fidel, PL Louisiana State Univ, Hlth Sci Ctr, Dept Microbiol Immunol & Parasitol, 1901 Perdido St, New Orleans, LA 70112 USA Louisiana State Univ 1901 Perdido St New Orleans LA USA 70112 SA
Citazione:
C. Steele et al., "Potential role for a carbohydrate moiety in anti-Candida activity of humanoral epithelial cells", INFEC IMMUN, 69(11), 2001, pp. 7091-7099

Abstract

Candida albicans is both a commensal and a pathogen at the oral mucosa. Although an intricate network of host defense mechanisms are expected for protection against oropharyngeal candidiasis, anti-Candida host defense mechanisms at the oral mucosa are poorly understood. Our laboratory recently showed that primary epithelial cells from human oral mucosa, as well as an oralepithelial cell line, inhibit the growth of blastoconidia. and/or hyphal phases of several Candida species in vitro with a requirement for cell contact and with no demonstrable role for soluble factors. In the present study,we show that oral epithelial cell-mediated anti-Candida activity is resistant to gamma-irradiation and is not mediated by phagocytosis, nitric oxide,hydrogen peroxide, and superoxide oxidative inhibitory pathways or by nonoxidative components such as soluble defensin and calprotectin peptides. In contrast, epithelial cell-mediated anti-Candida activity was sensitive to heat, paraformaldehyde fixation, and detergents, but these treatments were accompanied by a significant loss in epithelial cell viability. Treatments that removed existing membrane protein or lipid moieties in the presence or absence of protein synthesis inhibitors had no effect on epithelial cell inhibitory activity. In contrast, the epithelial cell-mediated anti-Candida activity was abrogated after treatment of the epithelial cells with periodicacid, suggesting a role for carbohydrates. Adherence of C albicans to oralepithelial cells was unaffected, indicating that the carbohydrate moiety is exclusively associated with the growth inhibition activity. Subsequent studies that evaluated specific membrane carbohydrate moieties, however, showed no role for sulfated polysaccharides, sialic acid residues, or glucose- and mannose-containing carbohydrates. These results suggest that oral epithelial cell-mediated anti-Candida activity occurs exclusively with viable epithelial cells through contact with C albicans by an as-yet-undefined carbohydrate moiety.

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Documento generato il 09/07/20 alle ore 16:39:11