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Titolo:
Dual role of the Leishmania major ribosomal protein S3a homologue in regulation of T- and B-cell activation
Autore:
Cordeiro-Da-Silva, A; Borges, MC; Guilvard, E; Ouaissi, A;
Indirizzi:
Univ Porto, Fac Pharm, Dept Biochem, Oporto, Portugal Univ Porto Oporto Portugal o, Fac Pharm, Dept Biochem, Oporto, Portugal Univ Porto, Inst Mol & Cellular Biol, Oporto, Portugal Univ Porto OportoPortugal , Inst Mol & Cellular Biol, Oporto, Portugal Ctr IRD Montpellier, IRD UR 008 Pathogenie & Trypanosomatides, F-34032 Rennes, France Ctr IRD Montpellier Rennes France F-34032 atides, F-34032 Rennes, France
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 11, volume: 69, anno: 2001,
pagine: 6588 - 6596
SICI:
0019-9567(200111)69:11<6588:DROTLM>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPERIMENTAL VISCERAL LEISHMANIASIS; SURFACE GLYCOPROTEIN; EXTENSIVE HOMOLOGY; ANTIGEN; SUPPRESSION; RESPONSES; AUTOANTIBODIES; ANTIBODY; GAMMA; INTERFERON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Cordeiro-Da-Silva, A Univ Porto, Fac Pharm, Dept Biochem, Rua Anibal Cunha164, Oporto, Portugal Univ Porto Rua Anibal Cunha 164 Oporto Portugal gal
Citazione:
A. Cordeiro-Da-Silva et al., "Dual role of the Leishmania major ribosomal protein S3a homologue in regulation of T- and B-cell activation", INFEC IMMUN, 69(11), 2001, pp. 6588-6596

Abstract

We have recently characterized a novel Leishmania major gene encoding a polypeptide of 30 kDa that was homologous to mammalian ribosomal protein S3a and was named LmS3a-related protein (LmS3arp). The protein was found to be expressed by all the Leishmania species so far examined (L. infantum, L. amazonensis, and L. mexicana). In the present study we have extended our approach to the analysis of LmS3arp activity on T- and B-cell functions in a murine model. The results presented in this report show that LmS3arp plays a dual role in the regulation of T- and B-cell reactivity. Indeed, we found that injection of the LmS3arp recombinant protein (rLmS3arp) into BALB/c mice induces preferential activation of B cells, as shown by the following criteria: (i) increased expression of CD69 molecules on immunoglobulin M (IgM)-secreting spleen cells, (ii) a considerable increase of IgM-secreting B cells, and (iii) elevated levels of IgM antibodies in the sera of injected animals. Moreover, the IgM antibodies are not specific to the Leishmania antigens but preferentially recognize heterologous antigens like myosin, thyroglobulin, DNA, and keyhole limpet hemocyanin. Furthermore, the strong polyclonal expansion of nonspecific, non-parasite-directed B-cell clones induced by rLmS3arp is concomitant with a marked inhibition of T-cell proliferation. Analysis of cytokine production revealed a significant downregulation of gamma interferon, interleukin-2 (IL-2), and IL-12 secretion. Taken together, our data suggest that rLmS3arp, through direct or indirect action toward B and T cells and cytokine secretion, could participate in the immunoregulatory processes that play a role in the balance of the Th1 and Th2 immune response.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 19:42:46