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Titolo:
Identification of membrane calcium channels essential for cytoplasmic and nuclear calcium elevations induced by vascular endothelial growth factor inhuman endothelial cells
Autore:
Garnier-Raveaud, S; Usson, Y; Cand, F; Robert-Nicoud, M; Verdetti, J; Faury, G;
Indirizzi:
Univ Grenoble 1, Grp Electrophysiol Mol, Lab Bioenerget Fondamentale & Appl, F-38041 Grenoble 09, France Univ Grenoble 1 Grenoble France 09 e & Appl, F-38041 Grenoble 09, France Univ Grenoble 1, INSERM, U309, F-38041 Grenoble, France Univ Grenoble 1 Grenoble France F-38041 , U309, F-38041 Grenoble, France Univ Grenoble 1, Inst A Bonniot, Lab Dynam Org Genome, F-38041 Grenoble 09, France Univ Grenoble 1 Grenoble France 09 g Genome, F-38041 Grenoble 09, France
Titolo Testata:
GROWTH FACTORS
fascicolo: 1, volume: 19, anno: 2001,
pagine: 35 - 48
SICI:
0897-7194(2001)19:1<35:IOMCCE>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR TYROSINE KINASES; ION CHANNELS; IN-VITRO; ANGIOGENESIS; CA2+; STIMULATION; EXPRESSION; DISTINCT; PROTEINS; INCREASE;
Keywords:
vascular endothelial growth factor; human umbilical venous endothelial cells; calcium channels; nucleus; proliferation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Faury, G Univ Grenoble 1, Grp Electrophysiol Mol, Lab Bioenerget Fondamentale & Appl, BP 53X, F-38041 Grenoble 09, France Univ Grenoble 1 BP 53X Grenoble France 09 41 Grenoble 09, France
Citazione:
S. Garnier-Raveaud et al., "Identification of membrane calcium channels essential for cytoplasmic and nuclear calcium elevations induced by vascular endothelial growth factor inhuman endothelial cells", GROW FACTOR, 19(1), 2001, pp. 35-48

Abstract

Vascular endothelial growth factor (VEGF) is mitogenic for endothelial cells and has been shown to induce angiogenesis and endothelial cell migrationthrough stimulation of endothelial tyrosine-kinase receptors. Here, using confocal. microscopy and the patch-damp technique on endothelial cells, membrane permeability to calcium as wen as cytoplasmic and nuclear free calcium levels have been investigated in the first stages of tyrosine-kinase receptor activation by VEGF. VEGF (0.5 nM) as well, as inositol trisphosphate (IP3) induced an activation of membrane calcium-permeable channels exhibiting a similar low conductance in the range of 10 pS. The VEGF-triggered activation of these calcium channels, mediated by IP3 and involving the intracellular calcium stores, results in an increase in both cytoplasmic and nuclear calcium levels in endothelial. cells, potentially modulating gene expression. Finally, the effect of Ni2+, a calcium channel blocker, on endothelialcell proliferation has been studied. The results show that inhibition of extracellular calcium influx significantly inhibits VEGF-induced cell proliferation. In the process of cell stimulation by VEGF, and possibly by other growth factors, activation of calcium channels could then be a key step in calcium-regulated gene expression and cell activation. These results suggest that the use of calcium channel blockers could be a novel way of prevention or reversion of VEGF-induced tumoral angiogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 17:02:14