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Titolo:
A novel rat dentin mRNA coding on ly for dentin sialoprotein
Autore:
Ritchie, HH; Li, XR;
Indirizzi:
Univ Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 & Endodont, Ann Arbor, MI 48109 USA
Titolo Testata:
EUROPEAN JOURNAL OF ORAL SCIENCES
fascicolo: 5, volume: 109, anno: 2001,
pagine: 342 - 347
SICI:
0909-8836(200110)109:5<342:ANRDMC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CASEIN KINASE-I; SEQUENCE DETERMINATION; SUBSTRATE DETERMINANTS; GENE; PHOSPHOPROTEIN; PHOSPHOPHORYN; PROTEINS; DSP; TRANSCRIPTS; CLONING;
Keywords:
dentin sialoprotein; multiple transcripts; DSP isoform; dentin mineralization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Ritchie, HH Univ Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, 1011 North Univ, Ann Arbor, MI 48109 USA Univ Michigan 1011 North Univ Ann Arbor MI USA 48109 48109 USA
Citazione:
H.H. Ritchie e X.R. Li, "A novel rat dentin mRNA coding on ly for dentin sialoprotein", EUR J OR SC, 109(5), 2001, pp. 342-347

Abstract

Dentin sialoprotein (DSP) is a major glycoprotein present in the mineralized dentin matrix that is expressed mainly by young and mature odontoblasts. Mutations in the DSP coding regions are linked to Dentinogenesis imperfecta I and II, indicating the importance of DSP in tooth formation. Previous studies have identified multiple mRNA transcripts in dentin that code for both DSP and phosphophoryns (PPs). Using reverse transcriptase-polymerase chain reaction (RT-PCR) to characterize these mRNA transcripts, we have identified a cDNA that codes for DSP, but not PP. This cDNA codes for a protein with 324 amino acids, 303 amino acids being identical to the published rat DSP sequence. However, the subsequent 21 amino acids are unique to this cDNA. Based on the coding sequence, the core protein is predicted to have a pI = 4.24, a net charge of -34, and to contain four potential N-glycosylation sites and six potential sites for phosphorylation by casein kinase. That the corresponding mRNA was present in day 5 molar tooth germs was confirmed using RNA protection assays. These data, therefore, identify a novel transcript in rat tooth germs that codes only for DSP (designated as DSPII).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:37:58