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Titolo:
Estrogen receptors in human preadipocytes
Autore:
Joyner, JM; Hutley, LJ; Cameron, DP;
Indirizzi:
Princess Alexandra Hosp, Dept Endocrinol & Diabet, Woolloongabba, Qld 4102, Australia Princess Alexandra Hosp Woolloongabba Qld Australia 4102 4102, Australia
Titolo Testata:
ENDOCRINE
fascicolo: 2, volume: 15, anno: 2001,
pagine: 225 - 230
SICI:
1355-008X(200107)15:2<225:ERIHP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ADIPOSE-TISSUE; BODY-FAT DISTRIBUTION; BREAST-CANCER-CELLS; POSTMENOPAUSAL WOMEN; GLUCOCORTICOID RECEPTOR; SKIN FIBROBLASTS; STROMAL CELLS; MESSENGER-RNA; IDENTIFICATION; CULTURE;
Keywords:
preadipocyte; estrogen receptor alpha; estrogen receptor beta; adipose tissue;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Joyner, JM Princess Alexandra Hosp, Dept Endocrinol & Diabet, Ipswich Rd, Woolloongabba, Qld 4102, Australia Princess Alexandra Hosp Ipswich Rd Woolloongabba Qld Australia 4102
Citazione:
J.M. Joyner et al., "Estrogen receptors in human preadipocytes", ENDOCRINE, 15(2), 2001, pp. 225-230

Abstract

Estrogen influences regional adipose tissue distribution and the accompanying cardiovascular disease risk. To elucidate the mechanisms of this link further, we assessed whether human preadipocytes (PAs) expressed estrogen receptors (ERs) and whether there were any regional or gender differences in ER complement. Human PAs expressed the ER alpha gene but not ERP by reversetranscriptase-polymerase chain reaction, possessed ER alpha protein on Western blotting, and displayed specific 17 beta -estradiol (E-2) binding withcalculated dissociation constants of 0.78 nM, 0.96 nM, and 1.19 nM and maximal binding capacities of 9.3 fmol/mg, 14.6 fmol/ mg, and 18.2 fmol/mg from three whole cell binding assays. There were no regional differences in ERalpha complement for males or females. There were no gender differences inER alpha complement for subcutaneous or visceral samples. We conclude thatER alpha but not ERP is present in human PAs. This suggests that the effect of estrogen on adipose tissue deposition has a contribution from the direct effect of estrogen on human PAs via ER alpha.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 04:42:10