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Titolo:
Prolactin-stimulated X-linked inhibitor of apoptosis protein expression during S phase cell cycle progression in rat Nb2 lymphoma cells
Autore:
Krishnan, N; Buckley, DJ; Zhang, MY; Reed, JC; Buckley, AR;
Indirizzi:
Univ Cincinnati, Med Ctr, Coll Pharm, Cincinnati, OH 45267 USA Univ Cincinnati Cincinnati OH USA 45267 l Pharm, Cincinnati, OH 45267 USA Univ Cincinnati, Med Ctr, Dept Cellular & Mol Physiol, Cincinnati, OH 45267 USA Univ Cincinnati Cincinnati OH USA 45267 Physiol, Cincinnati, OH 45267 USA Burnham Inst, La Jolla, CA 92037 USA Burnham Inst La Jolla CA USA 92037Burnham Inst, La Jolla, CA 92037 USA
Titolo Testata:
ENDOCRINE
fascicolo: 2, volume: 15, anno: 2001,
pagine: 177 - 186
SICI:
1355-008X(200107)15:2<177:PXIOAP>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA-INDUCED APOPTOSIS; NF-KAPPA-B; ENDOTHELIAL-CELLS; NUCLEAR TRANSLOCATION; MYELOID-LEUKEMIA; SODIUM-BUTYRATE; RETINOIC ACID; IAP GENE; XIAP; INDUCTION;
Keywords:
prolactin; X-linked inhibitor of apoptosis; cell cycle; Nb2 lymphoma cells; apoptosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Buckley, AR Univ Cincinnati, Med Ctr, Coll Pharm, 3223 Eden Ave,POB 670004, Cincinnati, OH 45267 USA Univ Cincinnati 3223 Eden Ave,POB 670004 Cincinnati OH USA 45267
Citazione:
N. Krishnan et al., "Prolactin-stimulated X-linked inhibitor of apoptosis protein expression during S phase cell cycle progression in rat Nb2 lymphoma cells", ENDOCRINE, 15(2), 2001, pp. 177-186

Abstract

The rat Nb2 lymphoma is useful for studying prolactin (PRL) receptor signaling to mitogenesis and apoptosis suppression. Previous results showed thatPRL rapidly induced expression of several apoptosis suppressor genes during the G1 phase of cell cycle in this model. The V linked inhibitor of apoptosis protein (XIAP) gene product acts to suppress apoptosis by direct inhibition of caspases. The present study was conducted to determine whether PRLalters XIAP expression in lactogen-dependent Nb2-11 or -independent Nb2-SFJCD1 cells. Stationary Nb2-11 cultures expressed detectable levels of an 8.9-kb XIAP transcript. PRL (20 ng/mL) stimulated its expression, reaching maximal levels within 12 h. Expression of XIAP was also evaluated in Nb2-SFJCD1 cells subsequent to treatment with differentiating agents (sodium butyrate [2 mM, 72 h], all trans-retinoic acid [10 muM, 72 h], or 1,25-dihydroxy-cholecalciferol [100 nM, 24 h]). PRL significantly increased XIAP expression in cells previously treated with these compounds. Further analysis revealed that PRL stimulated XIAP expression during S phase of the cell cycle. Todetermine whether XIAP suppressed apoptosis, its cDNA was stably transfected into Nb2-11 cells. Compared to controls, cells overexpressing XIAP exhibited substantially less DNA fragmentation when apoptosis was induced by PRLdeprivation or glucocorticoids. We conclude that PRL-stimulated XIAP expression likely serves to suppress apoptosis as cells progress through the later phases of the cell cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 19:29:25