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Titolo:
Effect of estradiol, diethylstilbestrol, and resveratrol on F0F1-ATPase activity from mitochondrial preparations of rat heart, liver, and brain
Autore:
Kipp, JL; Ramirez, VD;
Indirizzi:
Univ Illinois, Dept Mol & Integrat Physiol, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 Integrat Physiol, Urbana, IL 61801 USA
Titolo Testata:
ENDOCRINE
fascicolo: 2, volume: 15, anno: 2001,
pagine: 165 - 175
SICI:
1355-008X(200107)15:2<165:EOEDAR>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSITIVITY-CONFERRING PROTEIN; ATP SYNTHASE; PERMEABILITY TRANSITION; ESTROGEN-RECEPTOR; STEROID-HORMONES; MEMBRANE ACTION; SERUM-ALBUMIN; CELL-LINE; BINDING; 17-BETA-ESTRADIOL;
Keywords:
estradiol; diethylstilbestrol; resveratrol; F0F1-ATPase/adenosine triphosphate synthase; mitochondria;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Kipp, JL Univ Illinois, Dept Mol & Integrat Physiol, 524 Burrill Hall,407 S GoodwinAve, Urbana, IL 61801 USA Univ Illinois 524 Burrill Hall,407 S Goodwin Ave Urbana IL USA 61801
Citazione:
J.L. Kipp e V.D. Ramirez, "Effect of estradiol, diethylstilbestrol, and resveratrol on F0F1-ATPase activity from mitochondrial preparations of rat heart, liver, and brain", ENDOCRINE, 15(2), 2001, pp. 165-175

Abstract

The question of whether estrogens or estrogen-like compounds would alter differentially the enzymatic activity of the F0F1-ATPase was addressed. Mitochondrial fractions of the liver, brain, and heart were obtained from adultmale rats and solubilized by digitonin. About 85 % of the adenosine triphosphate hydrolysis by these three preparations come from the mitochondrial F0F1-ATPase. The enzymatic activity differed in the following order: liver <brain < heart. A concentration of 13 nM estradiol stimulated the F0F1-ATPase activity in heart by 10% (p < 0.01), but not in liver or brain. 17 beta -estradiol competed off the binding of estradiol-17 beta -17-(O-carboxymethyl)oxime:I-125- labeled bovine serium albumin to mitochondrial preparationsof the heart, revealing two binding sites. Resveratrol inhibited the F0F1-ATPase activity in both heart and liver with an IC50 of 13-15 muM, which confirmed our previous report in preparations of brain. Lower doses (picomolar to nanomolar) of resveratrol stimulated the F0F1-ATPase activity in liverby 10% but not in heart. At 6.7 muM, diethylstilbestrol (DES) inhibited the F0F1-ATPase activity in the three preparations by 61-67%. This study demonstrates that estradiol activates rat heart mitochondrial F0F1-ATPase at physiologic concentrations and that the F0F1-ATPase activity is markedly different in rat liver, brain, and heart. In addition, estradiol, DES, and resveratrol alter the F0F1-ATPase activity selectively, probably via different mechanisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:41:27