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Titolo:
Gene gun-mediated oral mucosal transfer of interleukin 12 cDNA coupled with an irradiated melanoma vaccine in a hamster model: Successful treatment of oral melanoma and distant skin lesion
Autore:
Wang, J; Murakami, T; Hakamata, Y; Ajiki, T; Jinbu, Y; Akasaka, Y; Ohtsuki, M; Nakagawa, H; Kobayashi, E;
Indirizzi:
Jichi Med Sch, Dept Dermatol, Minami Kawachi, Tochigi 3290498, Japan JichiMed Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Ctr Mol Med, Div Organ Replacement Res, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Dept Oral Surg, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan
Titolo Testata:
CANCER GENE THERAPY
fascicolo: 10, volume: 8, anno: 2001,
pagine: 705 - 712
SICI:
0929-1903(200110)8:10<705:GGOMTO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
STIMULATORY FACTOR INTERLEUKIN-12; T-CELLS; IN-VIVO; ANTITUMOR IMMUNITY; RECOMBINANT IL-12; LANGERHANS CELLS; MURINE TUMORS; CYTOKINE; GAMMA; MICE;
Keywords:
oral melanoma; interleukin 12; gene expression; gene gun; gene therapy; antitumor vaccination;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Murakami, T Jichi Med Sch, Dept Dermatol, 3311-1 Yakushiji, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch 3311-1 Yakushiji Minami Kawachi Tochigi Japan 3290498
Citazione:
J. Wang et al., "Gene gun-mediated oral mucosal transfer of interleukin 12 cDNA coupled with an irradiated melanoma vaccine in a hamster model: Successful treatment of oral melanoma and distant skin lesion", CANC GENE T, 8(10), 2001, pp. 705-712

Abstract

Malignant melanoma involving the oral cavity has a highly metastatic potential. Curative surgery is required to resect extensive oral tissues and often results in dysfunction as well as a severe cosmetic deformity in patients with the disease. An alternative technology for the local and sustained delivery of cytokines for cancer immunotherapy has been shown to induce tumor regression, suppression of metastasis, and development of systemic antitumor immunity. However, local immunization of the oral cavity has not previously been studied. In this study, we examined the efficacy of particle-mediated oral gene transfer on luciferase and green fluorescent protein production. The results showed that these proteins were more significantly expressed in oral mucosa than the skin, stomach, liver, and muscle. Using an established oral melanoma model in hamsters, particle-mediated oral gene gun therapy with interleukin (IL) 12 cDNA was then conducted. The results indicated that direct bombardment of mouse IL-12 cDNA suppressed tumor formation and improved the survival rate. The skin tumor model created by inoculation of melanoma cells was also significantly inhibited by the oral bombardment of IL-12 cDNA coupled with an irradiated melanoma vaccine administrated to the oral mucosa, compared to treatment with a percutaneous vaccine. IL-12 gene gun therapy, combined with an oral mucosal vaccine, induced interferon-gamma, mRNA expression in the host spleen for a long time. These results suggest that immunization of oral mucosa may induce systemic antitumor immunity more efficiently than immunization of the skin and that oral mucosa may be one of the most suitable tissues for cancer gene therapy by means of particle-mediated gene transfer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 21:55:10