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Titolo:
Enhanced anti-tumor effects of herpes simplex virus thymidine kinase/ganciclovir system by codelivering monocyte chemoattractant protein-1 in hepatocellular carcinoma
Autore:
Sakai, Y; Kaneko, S; Nakamoto, Y; Kagaya, T; Mukaida, N; Kobayashi, K;
Indirizzi:
Kanazawa Univ, Sch Med, Dept Internal Med 1, Kanazawa, Ishikawa 9208641, Japan Kanazawa Univ Kanazawa Ishikawa Japan 9208641 wa, Ishikawa 9208641, Japan Kanazawa Univ, Canc Res Inst, Div Mol Bioregulat, Kanazawa, Ishikawa 9208641, Japan Kanazawa Univ Kanazawa Ishikawa Japan 9208641 wa, Ishikawa 9208641, Japan
Titolo Testata:
CANCER GENE THERAPY
fascicolo: 10, volume: 8, anno: 2001,
pagine: 695 - 704
SICI:
0929-1903(200110)8:10<695:EAEOHS>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; P53 GENE-THERAPY; ADENOVIRUS-MEDIATED TRANSFER; EXPERIMENTAL BRAIN-TUMORS; P-GLYCOPROTEIN ANTIBODY; HUMAN LUNG-CANCER; IN-VIVO; RECOMBINANT ADENOVIRUS; CHEMOTACTIC PROTEIN-1; CRE RECOMBINASE;
Keywords:
monocyte chemoattractant protein-1; suicide gene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Kaneko, S Kanazawa Univ, Sch Med, Dept Internal Med 1, 13-1 Takara Machi, Kanazawa, Ishikawa 9208641, Japan Kanazawa Univ 13-1 Takara Machi Kanazawa Ishikawa Japan 9208641
Citazione:
Y. Sakai et al., "Enhanced anti-tumor effects of herpes simplex virus thymidine kinase/ganciclovir system by codelivering monocyte chemoattractant protein-1 in hepatocellular carcinoma", CANC GENE T, 8(10), 2001, pp. 695-704

Abstract

The therapeutic efficacy of herpes simplex virus thymidine kinase/ganciclovir (HSV-tk/GCV) system in many types of tumors is unsatisfactory due to the insufficient spread of gene transfer and insufficient cell killing. In the current study, we investigated whether adenovirally delivered monocyte chemoattractant protein (MCP)-1 potentiates the antitumor effects of the HSV-tk/GCV system in hepatocellular carcinoma (HCC) cells. Subcutaneous tumor foci of the human HCC cell line, HuH7, established in athymic mice were directly transduced with a recombinant adenovirus (rAd) harboring an HSV-tk gene driven by a human alpha -fetoprotein promoter, followed by GCV administration. Subsequently, another rAd expressing MCP-1 under the universal CAG promoter was injected. The growth of tumors was markedly suppressed by codelivering HSV-tk and MCP-1 genes compared to that by either HSV-tk/GCV or MCP-1 delivery. in the tumor tissues, monocyte/macrophage infiltration was detected immunohistochemically, The antitumor effects of the rAd expressing MCP-1 were markedly reduced by the administration of carrageenan, a compound known to inactivate macrophage. These results indicate that adenovirally delivered MCP-1 enhanced the antitumor effects of the HSV-tk/GCV system synergistically by recruitment/activation of macrophages in tumor tissues, suggesting an effective immunotherapy for HCC and other lineages of tumors when used adjuvantly with a suicide gene.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:15:01