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Titolo:
Prostate-specific targeting using PSA promoter-based lentiviral vectors
Autore:
Yu, D; Chen, DL; Chiu, C; Razmazma, B; Chow, YH; Pang, S;
Indirizzi:
Univ Calif Los Angeles, Sch Dent, Div Oral Biol & Med, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Univ Calif Los Angeles, Sch Dent, Inst Dent, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
CANCER GENE THERAPY
fascicolo: 9, volume: 8, anno: 2001,
pagine: 628 - 635
SICI:
0929-1903(200109)8:9<628:PTUPPL>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIGEN GENE; TISSUE-SPECIFICITY; ANDROGEN RECEPTOR; CANCER-CELLS; EXPRESSION; CARCINOMA; IDENTIFICATION; MANAGEMENT; TOXICITY; ENHANCER;
Keywords:
PSA; PSA promoter; DTA; prostate cancer; tissue-specific;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Pang, S Univ Calif Los Angeles, Sch Dent, Div Oral Biol & Med, 10833 Le Conte Ave,Los Angeles, CA 90095 USA Univ Calif Los Angeles 10833 Le Conte AveLos Angeles CA USA 90095
Citazione:
D. Yu et al., "Prostate-specific targeting using PSA promoter-based lentiviral vectors", CANC GENE T, 8(9), 2001, pp. 628-635

Abstract

The prostate-specific antigen (PSA) promoter is known to be highly tissue specific. Although its tissue specificity has been confirmed, its efficiency of gene transcription is significantly lower compared to known nonspecific viral promoters, These lower levels of promoter activity therefore pose aproblem when developing an efficacious gene vector for prostate cancer gene therapy. Thus, selecting an appropriate therapeutic gene and vector system to carry the gene driven by the PSA promoter (PSAP) is important. In the studies described here, a human immunodeficiency virus (HIV)-1-based lentiviral vector carrying either the enhanced green fluorescent protein (EGFP) reporter or the diphtheria toxin A (DTA) gene was constructed. The results demonstrate that the PSA promoter in a lentiviral vector drives genes in prostate cells with satisfactory efficacy and specificity. The tissue-specificexpression of the DTA protein efficiently eradicates LNCaP prostate cel Isin culture. We also infected prostate cancer cel Is and control cells carried by nude mice with the EGFP lentiviral vector. Significant numbers of EGFP-positive LNCaP cells were detected in all the mice bearing these tumors,but no EGFP-positive control cells were detected in any other mouse tissue. The high levels of expression in prostate cells, compared with the low levels of background expression in other cells, show that the PSAP-lentiviralvector could be a potential useful tool for gene therapy of metastatic prostate cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:51:19