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Titolo:
A simple and sensitive test for field diagnosis of post kala-azar dermal leishmaniasis
Autore:
Salotra, P; Sreenivas, G; Ramesh, V; Sundar, S;
Indirizzi:
ICMR, Inst Pathol, Mol Biol Lab, New Delhi 110029, India ICMR New Delhi India 110029 athol, Mol Biol Lab, New Delhi 110029, India Safdarjang Hosp, Dept Dermatol, New Delhi, India Safdarjang Hosp New Delhi India g Hosp, Dept Dermatol, New Delhi, India Banaras Hindu Univ, Inst Med Sci, Kala Azar Med Res Ctr, Varanasi 221005, Uttar Pradesh, India Banaras Hindu Univ Varanasi Uttar Pradesh India 221005 tar Pradesh, India
Titolo Testata:
BRITISH JOURNAL OF DERMATOLOGY
fascicolo: 4, volume: 145, anno: 2001,
pagine: 630 - 632
SICI:
0007-0963(200110)145:4<630:ASASTF>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
VISCERAL LEISHMANIASIS;
Keywords:
k39 antigen; postkala-azar dermal leishmaniasis; serodiagnosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Salotra, P ICMR, Inst Pathol, Mol Biol Lab, Safdarjung Hosp Campus,Post Box 4909, NewDelhi 110029, India ICMR Safdarjung Hosp Campus,Post Box 4909 New Delhi India 110029
Citazione:
P. Salotra et al., "A simple and sensitive test for field diagnosis of post kala-azar dermal leishmaniasis", BR J DERM, 145(4), 2001, pp. 630-632

Abstract

Background Current methods for diagnosis of post kala-azar dermal leishmaniasis (PKDL) do not offer adequate sensitivity and specificity. Objectives To develop a simple and sensitive test for field diagnosis of PKDL. Methods Immunochromatographic nitrocellulose strips precoated with recombinant k39 antigen were evaluated for the detection of circulating antibodiesto leishmanial k39 in PKDL sera. A drop of serum applied to the strip followed by buffer led to the development of two visible bands indicating the presence of anti-k39 IgG. Results The strip test was able to detect cases of PKDL with 91% sensitivity. The specificity of the test was evaluated using controls with other skin diseases, other common infections and healthy persons from endemic and non-endemic regions. Of 125 controls examined, all were negative on the striptest, indicating 100% specificity of the test. Conclusions The immunochromatographic nitrocellulose strips provide a non-invasive. rapid and accurate method for diagnosing PKDL.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 07:48:03