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Titolo:
Parathyroid hormone-stimulated resorption in calvaria cultured in serum-free medium is enhanced by the calcium-mobilizing activity of 1,25-dihydroxyvitamin D-3
Autore:
Li, W; Farach-Carson, MC;
Indirizzi:
Univ Delaware, Dept Biol Sci, Newark, DE 19716 USA Univ Delaware Newark DE USA 19716 re, Dept Biol Sci, Newark, DE 19716 USA
Titolo Testata:
BONE
fascicolo: 3, volume: 29, anno: 2001,
pagine: 231 - 235
SICI:
8756-3282(200109)29:3<231:PHRICC>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
VITAMIN-D METABOLITES; BONE-RESORPTION; OSTEOSARCOMA CELLS; ORGAN-CULTURE; ANALOG; HOMEOSTASIS; ACTIVATION; RECEPTOR; CURRENTS; INVITRO;
Keywords:
1,25-dihydroxyvitamin D-3; vitamin D analogs; parathyroid hormone (PTH); bone resorption; osteoblasts; calcium channels;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Farach-Carson, MC Univ Delaware, Dept Biol Sci, Room 304 Wolf Hall, Newark, DE 19716 USA Univ Delaware Room 304 Wolf Hall Newark DE USA 19716 USA
Citazione:
W. Li e M.C. Farach-Carson, "Parathyroid hormone-stimulated resorption in calvaria cultured in serum-free medium is enhanced by the calcium-mobilizing activity of 1,25-dihydroxyvitamin D-3", BONE, 29(3), 2001, pp. 231-235

Abstract

1,25(OH)(2)D-3 enhances parathyroid hormone (PTH)-induced Ca2+ signaling in osteoblasts by activating plasma membrane voltage-sensitive Ca2+ channels(VSCCs). The ability of 1,25(OH)(2)D-3 or the VSCC-activating analog AT (25-hydroxy-16-ene-23-yne-D-3) to enhance parathyroid hormone-stimulated Ca-45(2+) release from cultured new-born rat calvaria was measured. Analog BT (1,24-dihydroxy-22-ene-24-cyclopropyl-D-3), that does not mobilize Ca2+, also was tested along with PTH. Control experiments were performed with and without PTH and with and without serum. Individual calvaria labeled in utero with Ca-45(2+) were cultivated in serum-free medium on filters at the medium/air interface of 24-well culture plates and Ca-45(2+) release followed over 72 h. The results demonstrated that 1,25(OH)(2)D-3 and the Ca2+-mobilizing analog, AT, but not the nuclear receptor-binding analog, BT, enhanced PTH-stimulated Ca-45(2+) release under serum-free conditions. This enhancement effect of the secosteroids was not evident in the presence of 10% fetal calf serum. The effect of analog AT was faster than that of 1,25(OH)(2)D-3. Nitrendipine, a specific L-type VSCC blocker, attenuated enhancement by vitamin D compounds, indicating that the high-threshold L-type VSCC is a molecular transducer of costimulation. These results emphasize the synergy between the calcitropic hormones 1,25(OH)(2)D-3 and PTH in cultures containing osteoblasts and osteoclasts, and suggest that the Ca2+-mobilizing activity of 1,25(OH)(2)D-3 enhances Ca2+ release from bone. (Bone 29:231-235; 2001) (C) 2001 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 15:41:17