Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Role of CFTR's PDZ1-binding domain, NBF1 and Cl- conductance in inhibitionof epithelial Na+ channels in Xenopus oocytes
Autore:
Boucherot, A; Schreiber, R; Kunzelmann, K;
Indirizzi:
Univ Queensland, Dept Physiol & Pharmacol, Sch Biomed Sci, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 Brisbane, Qld 4072, Australia
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
fascicolo: 1, volume: 1515, anno: 2001,
pagine: 64 - 71
SICI:
0005-2736(20011101)1515:1<64:ROCPDN>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PDZ-INTERACTING DOMAIN; PROTEIN-KINASE-A; CYSTIC-FIBROSIS; FUNCTIONAL SIMILARITIES; MEDIATED INHIBITION; AIRWAY EPITHELIUM; REGULATOR CFTR; BINDING; ASSOCIATION; ACTIVATION;
Keywords:
cystic fibrosis transmembrane conductance regulator; epithelial Na+ channel; Xenopus oocyte; mouse trachea; cystic fibrosis; PDZ1; Na+/H+-exchange regulator factors; tyrosine kinase activating protein; cAMP; epithelial transport; nucleotide binding fold;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Kunzelmann, K Univ Queensland, Dept Physiol & Pharmacol, Sch Biomed Sci, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 4072, Australia
Citazione:
A. Boucherot et al., "Role of CFTR's PDZ1-binding domain, NBF1 and Cl- conductance in inhibitionof epithelial Na+ channels in Xenopus oocytes", BBA-BIOMEMB, 1515(1), 2001, pp. 64-71

Abstract

The cystic fibrosis transmembrane conductance regulator (CFTR) inhibits epithelial Na+ channels (ENaC). Evidence has accumulated that both Cl- transport through CFTR Cl- channels and the first nucleotide binding domain (NBF1) of CFTR are crucial for inhibition of ENaC. A PDZ binding domain (PDZ-BD)at the C-terminal end links CFTR to scaffolding and cytoskeletal proteins,which have been suggested to play an important role in activation of CFTR and eventually inhibition of ENaC. We eliminated the PDZ-BD of CFTR and coexpressed Na+/H+-exchange regulator factors together with CFTR and ENaC. Theresults do not support a role of PDZ-BD in inhibition of ENaC by CFTR. However, inhibition of ENaC was closely linked to Cl- currents generated by CFTR and was observed in the presence of Cl-, I- or Br- but not gluconate. Therefore, functional NBF1 and Cl- transport are required for inhibition of ENaC in Xenopus oocytes, while the PDZ-BD is not essential. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 15:24:42