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Titolo:
Uptake of oxidized low-density lipoprotein in a THP-1 cell line lacking scavenger receptor A
Autore:
Sugano, R; Yamamura, T; Harada-Shiba, M; Miyake, Y; Yamamoto, A;
Indirizzi:
Natl Cardiovasc Ctr, Res Inst, Dept Etiol & Pathophysiol, Osaka 5658565, Japan Natl Cardiovasc Ctr Osaka Japan 5658565 thophysiol, Osaka 5658565, Japan
Titolo Testata:
ATHEROSCLEROSIS
fascicolo: 2, volume: 158, anno: 2001,
pagine: 351 - 357
SICI:
0021-9150(200110)158:2<351:UOOLLI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOMOZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA; NONHUMAN PRIMATE; MEMBRANE-PROTEIN; HUMAN CD68; MACROPHAGE; CHOLESTEROL; EXPRESSION; CD36; DEGRADATION; FIBROBLASTS;
Keywords:
acetylated LDL; atherosclerosis; CD36; cholesterol; foam cell; macrophage; oxidized LDL; scavenger receptor; THP-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Yamamura, T Natl Cardiovasc Ctr, Res Inst, Dept Etiol & Pathophysiol, 5-7-1 Fujishiro Dai, Osaka 5658565, Japan Natl Cardiovasc Ctr 5-7-1 Fujishiro Dai Osaka Japan 5658565 n
Citazione:
R. Sugano et al., "Uptake of oxidized low-density lipoprotein in a THP-1 cell line lacking scavenger receptor A", ATHEROSCLER, 158(2), 2001, pp. 351-357

Abstract

We previously isolated THP-1 subtype cells (sTHP-1), a cell line that expresses scanty amounts of scavenger receptor A (ScR-A) and does not undergo foam cell formation when incubated with acetylated low-density lipoprotein (Ac-LDL). In this study, we investigated the accumulation of esterified cholesterol in sTHP-1 cells incubated with oxidized LDL (Ox-LDL), a physiologically modified lipoprotein in human. While sTHP-1 cells incubated with Ac-LDL accumulated only small amounts of esterified cholesterol, those incubatedwith Ox-LDL accumulated amounts similar to those accumulated by parent THP-1 (pTHP-1) cells. sTHP-1 cells expressed CD36 in amounts similar to the amounts expressed by pTHP-1 cells, and Ox-LDL was internalized through this CD36. The amount of accumulated esterified cholesterol was 73-81% of that accumulated in pTHP-1 cells expressing ScR-A. The levels of I-125-Ox-LDL binding, association, and degradation in sTHP-1 cells were 64-70% of the corresponding levels in pTHP-1 cells. In our experiments utilizing ScR-A-deficient sTHP-1 cells and a specific antibody against human CD36, most of the Ox-LDL interacted with the CD36 receptor. In addition, a substantial amount of Ox-LDL (28-42%) was bound and degraded by sTHP-1 macrophages when both of the two major scavenger receptors, ScR-A and CD36, were deficient or blocked. These results indicate that CD36 in macrophages plays an important role in foam cell formation by Ox-LDL, while additional scavenger receptor(s) maytake part in significant pathways of Ox-LDL uptake in macrophages. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:54:24