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Titolo:
Capillary electrophoresis with laser-induced fluorescence detection for chiral analysis and DNA binding studies of ruthenium(II) Tris-diimine complexes
Autore:
Martin, SE; Connatser, RM; Kane-Maguire, NAP; Wheeler, JF;
Indirizzi:
Furman Univ, Dept Chem, Greenville, SC 29613 USA Furman Univ Greenville SC USA 29613 , Dept Chem, Greenville, SC 29613 USA
Titolo Testata:
ANALYTICA CHIMICA ACTA
fascicolo: 1, volume: 445, anno: 2001,
pagine: 21 - 27
SICI:
0003-2670(20011025)445:1<21:CEWLFD>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
METAL-LIGAND COMPLEXES; PROTEIN HYDRODYNAMICS; METALLOINTERCALATORS; SEPARATION; IRON(II); MEKC;
Keywords:
capillary electrophoresis; laser-induced fluorescence; ruthenium complexes; DNA binding;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Wheeler, JF Furman Univ, Dept Chem, 3300 Poinsett Highway, Greenville, SC 29613 USA Furman Univ 3300 Poinsett Highway Greenville SC USA 29613 USA
Citazione:
S.E. Martin et al., "Capillary electrophoresis with laser-induced fluorescence detection for chiral analysis and DNA binding studies of ruthenium(II) Tris-diimine complexes", ANALYT CHIM, 445(1), 2001, pp. 21-27

Abstract

The separation of Ru(II) tris-diimine enantiomers was performed by chiral capillary electrophoresis (CE). A highly sensitive laser-induced fluorescence (LIF) detection system was employed for detection of transition metal complexes present in solution at the low micromolar range. An argon ion laser(457.9 nm, 0.15 mW) was used as an excitation source. The enantiomers of the [Ru(diimine)(3)](2+) complexes were separated using either potassium antimonyl-D-tartrate or dibenzoyl-L-tartrate (L-DBT) as chiral resolving agents. The concentration limit of detection (LOD) for the tris(1,10-phenanthroline) Ru(H) complex was 12.5 muM and the LOD for the tris(2,2'-bipyridine) ruthenium(II) complex was 1.0 muM. These limits represent an improvement in the overall LOD by three-orders of magnitude relative to that obtained via UV-VIS detection. The CE-LIF system was also used to assess the stereoselective interactions between tris(1,10-phenanthroline) Ru(II) complexes and B-form deoxyribonucleic acid (B-DNA). (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 16:48:13