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Titolo:
Basic electrical properties of in situ endothelial cells of small pulmonary arteries during postnatal development
Autore:
Olschewski, A; Olschewski, H; Brau, ME; Hempelmann, G; Vogel, W; Safronov, BV;
Indirizzi:
IBMC, P-4150180 Oporto, Portugal IBMC Oporto Portugal P-4150180IBMC, P-4150180 Oporto, Portugal Univ Giessen, Dept Physiol, Giessen, Germany Univ Giessen Giessen Germany iv Giessen, Dept Physiol, Giessen, Germany Univ Giessen, Dept Anaesthesiol & Intens Care Med, Giessen, Germany Univ Giessen Giessen Germany hesiol & Intens Care Med, Giessen, Germany Univ Giessen, Dept Internal Med, D-6300 Giessen, Germany Univ Giessen Giessen Germany D-6300 nternal Med, D-6300 Giessen, Germany
Titolo Testata:
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
fascicolo: 3, volume: 25, anno: 2001,
pagine: 285 - 290
SICI:
1044-1549(200109)25:3<285:BEPOIS>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CA2+-ACTIVATED K+ CHANNELS; GUINEA-PIG HEART; PATCH-CLAMP; VASCULAR ENDOTHELIUM; POTASSIUM CHANNELS; RELAXING FACTOR; CATION CHANNEL; ION CHANNELS; CONDUCTANCE; CALCIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Safronov, BV IBMC, Rua Campo Alegre 823, P-4150180 Oporto, Portugal IBMC Rua Campo Alegre 823 Oporto Portugal P-4150180 Portugal
Citazione:
A. Olschewski et al., "Basic electrical properties of in situ endothelial cells of small pulmonary arteries during postnatal development", AM J RESP C, 25(3), 2001, pp. 285-290

Abstract

Small pulmonary arteries are the major determinants of pulmonary artery pressure and vascular resistance. Their endothelium modulates pulmonary resistance, remodeling, and blood fluidity. We developed a method that provides access to the luminal surface of small pulmonary arteries of rat and allowsthe patch-clamp study of electrical properties of in situ endothelium. At birth, the membrane was predominantly permeable for K+, showing a resting potential of -70 mV. This conductance was not voltage-dependent and was insensitive to standard blockers of K channels such as tetraethylammonium, charybdotoxin, and 4-aminopyridine. The first 22 d of development were accompanied by an additional expression of a Cl- conductance, increasing membrane potential to -45 mV. Acidosis reduced KI conductance and depolarized the membrane, whereas alkalosis resulted in hyperpolarization. Two-electrode recordings revealed tight electrical coupling (83%) between neighboring cells inthe circumferential direction of the artery. The electrotonic length constant for endothelium was 13.3 mum, indicating that most cells in one cross section of a small artery are well coupled. Thus, the resting membrane conductances in small pulmonary artery endothelial cells change with postnatal development and are modulated by pH.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 18:23:11