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Titolo:
Physiological effect of circulating glucagon on the hepatic membrane potential
Autore:
Lutz, TA; Estermann, A; Geary, N; Scharrer, E;
Indirizzi:
Univ Zurich, Inst Vet Physiol, CH-8057 Zurich, Switzerland Univ Zurich Zurich Switzerland CH-8057 siol, CH-8057 Zurich, Switzerland Cornell Univ, Weill Med Coll, White Plains, NY 10605 USA Cornell Univ White Plains NY USA 10605 d Coll, White Plains, NY 10605 USA New York Presbyterian Hosp, EW Bourne Behav Res Lab, White Plains, NY 10605 USA New York Presbyterian Hosp White Plains NY USA 10605 Plains, NY 10605 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
fascicolo: 5, volume: 281, anno: 2001,
pagine: R1540 - R1544
SICI:
0363-6119(200111)281:5<R1540:PEOCGO>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPONTANEOUS MEAL SIZE; FATTY-ACID OXIDATION; PANCREATIC GLUCAGON; FOOD-INTAKE; POSTPRANDIAL SATIETY; RAT-LIVER; INTRAPERITONEAL INJECTION; PORTAL INFUSION; CELL-MEMBRANE; IN-VIVO;
Keywords:
food intake; glucagon antiserum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Lutz, TA Univ Zurich, Inst Vet Physiol, Winterthurerstr 260, CH-8057 Zurich, Switzerland Univ Zurich Winterthurerstr 260 Zurich Switzerland CH-8057 rland
Citazione:
T.A. Lutz et al., "Physiological effect of circulating glucagon on the hepatic membrane potential", AM J P-REG, 281(5), 2001, pp. R1540-R1544

Abstract

The pancreatic hormone glucagon hyperpolarizes the liver cell membrane under various conditions. Here we investigated the physiological relevance of this effect by testing the influence of infusions of glucagon antiserum on the liver cell membrane potential in vivo. Intracellular microelectrode recordings of liver cells (up to 60/rat over 2 h) were done in anesthetized male rats. Livers were fixed in place, and recordings were done 10-30 min after intraperitoneal injections of glucagon or hepatic portal vein infusions of glucagon or specific polyclonal glucagon antibodies raised in rabbits. The isotonic lactose vehicle was used as a control for glucagon, and equal amounts of nonimmunized rabbit IgG were used as a control for glucagon antibodies. Intraperitoneal glucagon (400 mug/kg) hyperpolarized the liver cell membrane up to 12 mV, and intraportal glucagon (10 or 60 mug/kg) dose dependently hyperpolarized the liver cell membrane by 3-7 mV. Intraportal infusion of glucagon antiserum (in vitro binding capacity of 4 ng glucagon/rat) significantly depolarized the liver cell membrane by similar to2.5 mV. The effects of both glucagon and glucagon antiserum reversed after 60-90 min. Weconclude that glucagon is a physiologically important modulator of the liver cell membrane potential.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 00:19:04