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Titolo:
Antisense inhibition of Na+/Ca2+ exchange during anoxia/reoxygenation in ventricular myocytes
Autore:
Eigel, BN; Hadley, RW;
Indirizzi:
Univ Kentucky, Coll Med, Dept Mol & Biomed Pharmacol, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 d Pharmacol, Lexington, KY 40536 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 5, volume: 281, anno: 2001,
pagine: H2184 - H2190
SICI:
0363-6135(200111)281:5<H2184:AIONED>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
SODIUM-CALCIUM EXCHANGE; REPERFUSION-INDUCED INJURY; GUINEA-PIG HEART; CARDIAC MYOCYTES; NA+-CA2+ EXCHANGE; DEPENDENT REGULATION; INTRACELLULAR PH; REVERSE MODE; CELLS; REOXYGENATION;
Keywords:
hypoxia; calcium; heart; ischemia; sodium/calcium exchanger;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Eigel, BN Univ Kentucky, Coll Med, Dept Mol & Biomed Pharmacol, UKMC MS-375, Lexington, KY 40536 USA Univ Kentucky UKMC MS-375 Lexington KY USA 40536n, KY 40536 USA
Citazione:
B.N. Eigel e R.W. Hadley, "Antisense inhibition of Na+/Ca2+ exchange during anoxia/reoxygenation in ventricular myocytes", AM J P-HEAR, 281(5), 2001, pp. H2184-H2190

Abstract

This study investigated the role of the Na+/Ca2+ exchanger (NCX) in regulating cytosolic intracellular Ca2+ concentration ([Ca2+](i)) during anoxia/reoxygenation in guinea pig ventricular myocytes. The hypothesis that the NCX is the predominant mechanism mediating [Ca2+](i) overload in this model was tested through inhibition of NCX expression by an antisense oligonucleotide. Immunocytochemistry revealed that this antisense oligonucleotide, directed at the area around the start site of the guinea pig NCX1, specificallyreduced NCX expression in cultured adult myocytes by 90 +/-4%. Antisense treatment inhibited evoked NCX activity by 94 +/-3% and decreased the rise in [Ca2+](i) during anoxia/reoxygenation by 95 +/-3%. These data suggest that NCX is the predominant mechanism mediating Ca2+ overload during anoxia/reoxygenation in guinea-pig ventricular myocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 15:52:10