Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Differential inhibition by botulinum neurotoxin A of cotransmitters released from autonomic vasodilator neurons
Autore:
Morris, JL; Jobling, P; Gibbins, IL;
Indirizzi:
Flinders Univ S Australia, Dept Anat & Histol, Ctr Neurosci, Adelaide, SA 5001, Australia Flinders Univ S Australia Adelaide SA Australia 5001 , SA 5001, Australia
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 5, volume: 281, anno: 2001,
pagine: H2124 - H2132
SICI:
0363-6135(200111)281:5<H2124:DIBBNA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTESTINAL POLYPEPTIDE VIP; SUB-MANDIBULAR GLAND; PIG UTERINE ARTERY; TOXIN TYPE-A; NITRIC-OXIDE; BLOOD-FLOW; NEUROMUSCULAR-TRANSMISSION; CLOSTRIDIAL NEUROTOXINS; NEUROTRANSMITTER; EXOCYTOSIS;
Keywords:
pelvic neurons; neuropeptides; nitric oxide; acetylcholine; synaptosomal-associated protein of 25 kDa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Morris, JL Flinders Univ S Australia, Dept Anat & Histol, Ctr Neurosci, GPO Box 2100,Adelaide, SA 5001, Australia Flinders Univ S Australia GPO Box 2100 Adelaide SA Australia 5001
Citazione:
J.L. Morris et al., "Differential inhibition by botulinum neurotoxin A of cotransmitters released from autonomic vasodilator neurons", AM J P-HEAR, 281(5), 2001, pp. H2124-H2132

Abstract

The role of the soluble NSF attachment protein receptor (SNARE) protein complex in release of multiple cotransmitters from autonomic vasodilator neurons was examined in isolated segments of guinea pig uterine arteries treated with botulinum neurotoxin A (BoNTA; 50 nM). Western blotting of protein extracts from uterine arteries demonstrated partial cleavage of synaptosomal-associated protein of 25 kDa (SNAP-25) to a NH2-terminal fragment of similar to 24 kDa by BoNTA. BoNTA reduced the amplitude (by 70-80%) of isometriccontractions of arteries in response to repeated electrical stimulation ofsympathetic axons at 1 or 10 Hz. The amplitude of neurogenic relaxations mediated by neuronal nitric oxide (NO) was not affected by BoNTA, whereas the duration of peptide-mediated neurogenic relaxations to stimulation at 10 Hz was reduced (67% reduction in integrated responses). In contrast, presynaptic cholinergic inhibition of neurogenic relaxations was abolished by BoNTA. These results demonstrate that the SNARE complex has differential involvement in release of cotransmitters from the same autonomic neurons: NO release is not dependant on synaptic vesicle exocytosis, acetylcholine releasefrom small vesicles is highly dependant on the SNARE complex, and neuropeptide release from large vesicles involves SNARE proteins that may interact differently with regulatory factors such as calcium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 16:25:20