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Titolo:
Connexin45 gap junction channels in rat cerebral vascular smooth muscle cells
Autore:
Li, X; Simard, JM;
Indirizzi:
Univ Maryland, Sch Med, Dept Neurosurg, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 t Neurosurg, Baltimore, MD 21201 USA Univ Maryland, Sch Med, Dept Pathol, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 Dept Pathol, Baltimore, MD 21201 USA Univ Maryland, Sch Med, Dept Physiol, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 ept Physiol, Baltimore, MD 21201 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 5, volume: 281, anno: 2001,
pagine: H1890 - H1898
SICI:
0363-6135(200111)281:5<H1890:CGJCIR>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIPLE CONNEXINS; NITRIC-OXIDE; MOUSE; PERMEABILITY; CONDUCTANCE; ENDOTHELIUM; EXPRESSION; HEART; ARTERIOLES; RESPONSES;
Keywords:
gap junctions; vascular smooth muscle; patch clamp;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Simard, JM Univ Maryland, Sch Med, Dept Neurosurg, 22 S Greene St, Baltimore, MD 21201 USA Univ Maryland 22 S Greene St Baltimore MD USA 21201 D 21201 USA
Citazione:
X. Li e J.M. Simard, "Connexin45 gap junction channels in rat cerebral vascular smooth muscle cells", AM J P-HEAR, 281(5), 2001, pp. H1890-H1898

Abstract

Cells in blood vessel walls express connexin (Cx) 43, Cx40, and Cx37. We recently characterized gap junction channels in rat basilar artery smooth muscle cells and found features attributable not only to these three connexins but also to an unidentified connexin, including strong voltage dependenceand single channel conductance of 30-40 pS. Here, we report data consistent with identification of Cx45. Immunofluorescence using anti-human Cx45 andanti-mouse Cx45 antibodies revealed labeling between alpha -actin-positivecells, and RT-PCR of mRNA from arteries after endothelial destruction yielded amplicons exhibiting 90-98% identity with mouse Cx45 and human Cx45. Dual-perforated patch clamping was performed after exposure to oligopeptides that interfere with docking of Cx43, Cx40, or Cx45. Cell pairs pretreated with blocking peptides for Cx43 and Cx40 exhibited strongly voltage-dependent transjunctional conductances [voltage at which voltage-dependent conductance declines by one-half (V-1/2) = +/-18.9 mV] and small single channel conductances (31 pS), consistent with the presence of Cx45, whereas cell pairspretreated with blocking peptide for Cx45 exhibit weaker voltage-dependentconductances (V-1/2 = +/-37.9 mV), consistent with block of Cx45. Our datasuggest that Cx45 is transcribed, expressed, and forms functional gap junction channels in rat cerebral arterial smooth muscle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:05:57