Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Changes in the exocrine pancreas secondary to altered small intestinal function in the CF mouse
Autore:
De Lisle, RC; Isom, KS; Ziemer, D; Cotton, CU;
Indirizzi:
Univ Kansas, Sch Med, Dept Anat & Cell Biol, Med Ctr, Kansas City, KS 66160 USA Univ Kansas Kansas City KS USA 66160 , Med Ctr, Kansas City, KS 66160 USA Case Western Reserve Univ, Dept Pediat, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Case Western Reserve Univ, Dept Physiol & Biophys, Cleveland, OH 44106 USACase Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
fascicolo: 4, volume: 281, anno: 2001,
pagine: G899 - G906
SICI:
0193-1857(200110)281:4<G899:CITEPS>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYSTIC-FIBROSIS MICE; ACINAR-CELLS; BICARBONATE SECRETION; SULFATED PROTEIN; ZYMOGEN GRANULES; CFTR(-/-) MICE; EXPRESSION; MEMBRANE; GENE; CONDUCTANCE;
Keywords:
amylase; bicarbonate ion; adenosine 3 ',5 '-cyclic monophosphate; cystic fibrosis; Muclin; secretin; vasoactive intestinal peptide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: De Lisle, RC Univ Kansas, Sch Med, Dept Anat & Cell Biol, Med Ctr, Kansas City, KS 66160 USA Univ Kansas Kansas City KS USA 66160 nsas City, KS 66160 USA
Citazione:
R.C. De Lisle et al., "Changes in the exocrine pancreas secondary to altered small intestinal function in the CF mouse", AM J P-GAST, 281(4), 2001, pp. G899-G906

Abstract

The exocrine pancreas of the cystic fibrosis (CF) mouse (cftr(m1UNC)) is only mildly affected compared with the human disease, providing a useful model to study alterations in exocrine function. The CF mouse pancreas has similar to 50% of normal amylase levels and similar to 200% normal Muclin levels, the major sulfated glycoprotein of the pancreas. Protein biosynthetic rates and mRNA levels for amylase were not altered in CF compared with normal mice, and increases in Muclin biosynthesis and mRNA paralleled the increased protein content. Stimulated pancreatic amylase secretion in vitro and in vivo tended to be increased in CF mice but was not statistically significant compared with normal mice. We show for the first time that the CF mouseduodenum is abnormally acidic (normal intestinal pH = 6.47 +/-0.05; CF intestinal pH = 6.15 +/-0.07) and hypothesize that this may result in increased signaling to the exocrine pancreas. There were significant increases in CF intestinal mRNA levels for secretin (310% of normal, P<0.001) and vasoactive intestinal peptide (148% of normal, P<0.05). Furthermore, CF pancreaticcAMP levels were 147% of normal (P<0.01). These data suggest that the CF pancreas may be chronically stimulated by cAMP-mediated signals, which in turn may exacerbate protein plugging in the acinar/ductal lumen, believed to be the primary cause of destruction of the pancreas in CF.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 20:58:00