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Titolo:
Epidermal growth factor induces Egr-1 promoter activity in hepatocytes in vitro and in vivo
Autore:
Tsai, JC; Liu, LX; Zhang, J; Spokes, KC; Topper, JN; Aird, WC;
Indirizzi:
Beth Israel Deaconess Med Ctr, Dept Mol Med, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr Boston MA USA 02215 d, Boston, MA 02215 USA Stanford Univ, Sch Med, Dept Med, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 Med, Dept Med, Stanford, CA 94305 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
fascicolo: 5, volume: 281, anno: 2001,
pagine: G1271 - G1278
SICI:
0193-1857(200111)281:5<G1271:EGFIEP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERUM RESPONSE ELEMENTS; COLONY-STIMULATING FACTOR; PRIMARY RAT HEPATOCYTES; IMMEDIATE-EARLY GENE; ENDOTHELIAL-CELLS; TRANSGENIC MICE; C-FOS; TRANSCRIPTION FACTORS; SIGNAL-TRANSDUCTION; PARTIAL-HEPATECTOMY;
Keywords:
immediate-early genes; transgenic mice; HepG2 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Aird, WC Beth Israel Deaconess Med Ctr, Dept Mol Med, RW-663,330 BrooklineAve, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr RW-663,330 Brookline Ave Boston MA USA 02215
Citazione:
J.C. Tsai et al., "Epidermal growth factor induces Egr-1 promoter activity in hepatocytes in vitro and in vivo", AM J P-GAST, 281(5), 2001, pp. G1271-G1278

Abstract

Early growth response-1 (Egr-1) is a transcription factor that couples short-term changes in the extracellular milieu to long-term changes in gene expression. Under in vitro conditions, the Egr-1 gene has been shown to respond to many extracellular signals. In most cases, these findings have not been extended to the in vivo setting. The goal of the present study was to explore the role of epidermal growth factor (EGF) in mediating Egr-1 expression in hepatocytes under both in vitro and in vivo conditions. In HepG2 cells, Egr-1 protein and mRNA were upregulated in the presence of EGF. In stable transfections of HepG2 cells, a 1,200-bp Egr-1 promoter contained information for EGF response via a protein kinase C-independent, mitogen-activatedprotein kinase-dependent signaling pathway. A promoter region containing the two most proximal serum response elements was sufficient to transduce the EGF signal. In transgenic mice that carry the Egr-1 promoter coupled to the LacZ reporter gene, systemic delivery of EGF by intraperitoneal injection resulted in an induction of the endogenous Egr-1 gene and the Egr-1-lacZ transgene in hepatocytes. Together, these results suggest that the 1,200-bppromoter contains information for EGF response in hepatocytes both in vitro and in intact animals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/08/20 alle ore 19:56:15