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Titolo:
Chronic exposure to high leucine impairs glucose-induced insulin release by lowering the ATP-to-ADP ratio
Autore:
Anello, M; Ucciardello, V; Piro, S; Patane, G; Frittitta, L; Calabrese, V; Stella, AMG; Vigneri, R; Purrello, F; Rabuazzo, AM;
Indirizzi:
Osped Garibaldi, Inst Internal Med Endocrinol & Metab, Div Endocrinol, I-95123 Catania, Italy Osped Garibaldi Catania Italy I-95123 Endocrinol, I-95123 Catania, Italy Osped Garibaldi, S Signorelli Diabet Ctr, I-95123 Catania, Italy Osped Garibaldi Catania Italy I-95123 Diabet Ctr, I-95123 Catania, Italy Univ Catania, Fac Med, Dept Chem, Sect Biochem & Mol Biol, I-95123 Catania, Italy Univ Catania Catania Italy I-95123 em & Mol Biol, I-95123 Catania, Italy
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
fascicolo: 5, volume: 281, anno: 2001,
pagine: E1082 - E1087
SICI:
0193-1849(200111)281:5<E1082:CETHLI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSITIVE K+ CHANNELS; PANCREATIC BETA-CELLS; MOUSE B-CELLS; GLUTAMATE-DEHYDROGENASE; ADENINE-NUCLEOTIDES; FATTY-ACIDS; SECRETION; ISLETS; DESENSITIZATION; PATHOGENESIS;
Keywords:
adenosine triphosphate; adenosine diphosphate; beta-cell desensitization; amino acids; adenine nucleotides; adenosine triphosphate potassium channel-independent pathway;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Anello, M Osped Garibaldi, Inst Internal Med Endocrinol & Metab, Div Endocrinol, Piazza S Maria di Gesu, I-95123 Catania, Italy Osped Garibaldi Piazza S Maria di Gesu Catania Italy I-95123 ly
Citazione:
M. Anello et al., "Chronic exposure to high leucine impairs glucose-induced insulin release by lowering the ATP-to-ADP ratio", AM J P-ENDO, 281(5), 2001, pp. E1082-E1087

Abstract

Exposure of rat pancreatic islets to 20 mM leucine for 24 h reduced insulin release in response to glucose (16.7 and 22.2 mM). Insulin release was normal when the same islets were stimulated with leucine (40 mM) or glyburide(1 muM). To investigate the mechanisms responsible for the different effect of these secretagogues, we studied several steps of glucose-induced insulin secretion. Glucose utilization and oxidation rates in leucine-precultured islets were similar to those of control islets. Also, the ATP-sensitive K channel-independent pathway of glucose-stimulated insulin release, studied in the presence of 30 mM K+ and 250 muM diazoxide, was normal. In contrast, the ATP-to-ADP ratio after stimulation with 22.2 mM glucose was reduced in leucine-exposed islets with respect to control islets. The decrease of the ATP-to-ADP ratio was due to an increase of ADP levels. In conclusion, prolonged exposure of pancreatic islets to high leucine levels selectively impairs glusoce-induced insulin release. This secretory abnormality is associated with (and might be due to) a reduced ATP-to-ADP ratio. The abnormal plasma amino acid levels often present in obesity and diabetes may, therefore, affect pancreatic islet insulin secretion in these patients.

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Documento generato il 14/07/20 alle ore 05:45:47