Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of fasting on muscle mitochondrial energetics and fatty acid metabolism in Ucp3(-/-) and wild-type mice
Autore:
Bezaire, V; Hofmann, W; Kramer, JKG; Kozak, LP; Harper, ME;
Indirizzi:
Univ Ottawa, Fac Med, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada Univ Ottawa Ottawa ON Canada K1H 8M5 Immunol, Ottawa, ON K1H 8M5, Canada Agr & Agri Food Canada, Food Res Program, Guelph, ON N1G 5C9, Canada Agr &Agri Food Canada Guelph ON Canada N1G 5C9 uelph, ON N1G 5C9, Canada Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA Pennington Biomed ResCtr Baton Rouge LA USA 70808 on Rouge, LA 70808 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
fascicolo: 5, volume: 281, anno: 2001,
pagine: E975 - E982
SICI:
0193-1849(200111)281:5<E975:EOFOMM>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
UNCOUPLING PROTEIN-3 EXPRESSION; BROWN ADIPOSE-TISSUE; RAT SKELETAL-MUSCLE; PROTON LEAK; UCP1-DEFICIENT MICE; ENERGY-METABOLISM; THYROID-HORMONE; UP-REGULATION; UCP3; THERMOGENESIS;
Keywords:
uncoupling proteins; uncoupling protein-2; uncoupling protein-3; proton leak; mitochondria; skeletal muscle; thermogenesis; energy expenditure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Harper, ME Univ Ottawa, Fac Med, Dept Biochem Microbiol & Immunol, 451 Smyth Rd, Ottawa, ON K1H 8M5, Canada Univ Ottawa 451 Smyth Rd Ottawa ON CanadaK1H 8M5 H 8M5, Canada
Citazione:
V. Bezaire et al., "Effects of fasting on muscle mitochondrial energetics and fatty acid metabolism in Ucp3(-/-) and wild-type mice", AM J P-ENDO, 281(5), 2001, pp. E975-E982

Abstract

Uncoupling protein-3 (UCP3) is a mitochondrial carrier protein of as yet undefined physiological function. To elucidate characteristics of its function, we studied the effects of fasting on resting metabolic rate, respiratory quotient, muscle Ucp3 expression, and mitochondrial proton leak in wild-type and Ucp3(-/-) mice. Also analyzed were the fatty acid compositions of skeletal muscle mitochondria in fed and fasted Ucp3(-/-) and wild-type mice. In wild-type mice, fasting caused significant increases in Ucp3 (4-fold) and Ucp2 (2-fold) mRNA but did not significantly affect mitochondrial protonleak. State 4 oxygen consumption was not affected by fasting in either of the two groups. However, protonmotive force was consistently higher in mitochondria of Ucp3(-/-) animals (P = 0.03), and fasting further augmented protonmotive force in Ucp3(-/-) mice; there was no effect in wild-type mitochondria. Resting metabolic rates decreased with fasting in both groups. Ucp3(-/-) mice had higher respiratory quotients than wild-type mice in fed resting states, indicating impaired fatty acid oxidation. Altogether, results show that the fasting-induced increases in Ucp2 and Ucp3 do not correlate with increased mitochondrial proton leak but support a role for UCP3 in fatty acid metabolism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/06/20 alle ore 12:43:08