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Titolo:
Detection of clonal T-cell receptor-gamma gene rearrangement by PCR/temporal temperature gradient gel electrophoresis
Autore:
Zhu, D; Kadin, ME; Samoszuk, M;
Indirizzi:
Quest Diagnost, Nichols Inst, Dept Hematol Oncol, San Juan Capistrano, CA 92690 USA Quest Diagnost San Juan Capistrano CA USA 92690 Capistrano, CA 92690 USA Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Boston, MA USA Harvard Univ Boston MA USA Beth Israel Deaconess Med Ctr, Boston, MA USA
Titolo Testata:
AJCP. American journal of clinical pathology
fascicolo: 4, volume: 116, anno: 2001,
pagine: 527 - 534
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; EARLY MYCOSIS-FUNGOIDES; LYMPHOID NEOPLASMS; POINT MUTATIONS; MARKERS; LINEAGE;
Keywords:
T-cell lymphoma; polymerase chain reaction; PCR; temporal temperature gradient gel electrophoresis; TTGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Samoszuk, M Quest Diagnost, Nichols Inst, Dept Hematol Oncol, 33608 OrtegaHighway, San Juan Capistrano, CA 92690 USA Quest Diagnost 33608 Ortega Highway San Juan Capistrano CA USA 92690
Citazione:
D. Zhu et al., "Detection of clonal T-cell receptor-gamma gene rearrangement by PCR/temporal temperature gradient gel electrophoresis", AM J CLIN P, 116(4), 2001, pp. 527-534

Abstract

Limited combinatorial and junctional diversity in TCR-gamma gene rearrangement can result in amplification products that are difficult to interpret when analyzed by conventional gel electrophoresis methods that separate DNA based on size (polymerase chain reaction [PCR]/polyactylamide gel electrophoresis [PAGE]). We describe a simple approach to the detection of clonal TCR-gamma gene rearrangement using temporal temperature gradient gel electrophoresis (TTGE) that uses a gradual and uniform increase in the temperature of a constant denaturing gel to resolve different DNA molecules based on base pair composition. We tested 42 clinical specimens (30 blood specimens and 12 formalin-fixed paraffin-embedded tissues) for T-cell clonality by PCR/PAGE and PCR/TTGE. Concordant results were obtained in only 22 specimens (52%). Of the 20 discordant cases, IS samples were positive by TTGE and negative by PAGE. For all of the discordant cases, the TTGE yielded results thatcon-elated better with the clinical data than did the PAGE method. We conclude that PCR/TTGE is more accurate and easier to perform than current methods for detecting clonal populations of T cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 12:12:15