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Titolo:
Adjuvant effects of IL-1 beta, IL-2, IL-8, IL-15, IFN-alpha, IFN-gamma TGF-beta 4 and lymphotactin on DNA vaccination against Eimeria acervulina
Autore:
Min, WG; Lillehoj, HS; Burnside, J; Weining, KC; Staeheli, P; Zhu, JJ;
Indirizzi:
USDA, Systemat Lab, Anim & Nat Resources Inst, Beltsville, MD 20705 USA USDA Beltsville MD USA 20705 Nat Resources Inst, Beltsville, MD 20705 USA Univ Delaware, Dept Anim & Food Sci, Newark, DE 19717 USA Univ Delaware Newark DE USA 19717 t Anim & Food Sci, Newark, DE 19717 USA Univ Freiburg, Dept Virol, D-79008 Freiburg, Germany Univ Freiburg Freiburg Germany D-79008 Virol, D-79008 Freiburg, Germany
Titolo Testata:
VACCINE
fascicolo: 1-2, volume: 20, anno: 2001,
pagine: 267 - 274
SICI:
0264-410X(20011012)20:1-2<267:AEOIBI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHICKEN INTERFERON-GAMMA; INTESTINAL IMMUNE-RESPONSES; MONOCLONAL-ANTIBODIES; FUNCTIONAL-CHARACTERIZATION; CYTOKINE THERAPY; EXPRESSION; GENE; CLONING; PROTEIN; IMMUNIZATION;
Keywords:
chicken; cytokine; adjuvant; coccidiosis; DNA vaccine; Eimeria;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Lillehoj, HS USDA, Systemat Lab, Anim & Nat Resources Inst, BARC E,Bldg 1040, Beltsville, MD 20705 USA USDA BARC E,Bldg 1040 Beltsville MD USA 20705 e, MD 20705 USA
Citazione:
W.G. Min et al., "Adjuvant effects of IL-1 beta, IL-2, IL-8, IL-15, IFN-alpha, IFN-gamma TGF-beta 4 and lymphotactin on DNA vaccination against Eimeria acervulina", VACCINE, 20(1-2), 2001, pp. 267-274

Abstract

Eight chicken cytokine genes (IL-1 beta, IL-2, IL-8, IL-15, IFN-alpha, IFN-gamma, TGF-beta4, lymphotactin) were evaluated for their adjuvant effect on a suboptimal dose of an Eimeria DNA vaccine carrying the 3-1E parasite gene (pcDNA3-1E). Chickens were given two subcutaneous injections with 50 mugof the pcDNA3-1E vaccine plus a cytokine expression plasmid 2 weeks apart and challenged with Eimeria acervulina 1 week later. IFN-alpha (1 mug) or 10 mug of lymphotactin expressing plasmids, when given simultaneously with the pcDNA3-1E vaccine, significantly protected against body weight loss induced by E. acervulina. Parasite replication was significantly reduced in chickens given the pcDNA3-1E vaccine along with 10 mug of the IL-8, lymphotactin, IFN-gamma, IL-15, TGF-beta4, or IL-1 beta plasmids compared with chickens given the pcDNA3-1E vaccine alone. Flow cytometric analysis of duodenum intraepithelial lymphocytes showed chickens that received the pcDNA3-1E vaccine simultaneously with the IL-8 or IL-15 genes had significantly increased CD3(+) cells compared with vaccination using pcDNA3-1E alone or in combination with the other cytokine genes tested. These results indicate that thetype and the dose of cytokine genes injected into chickens influence the quality of the local immune response to DNA vaccination against coccidiosis. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 09:19:16