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Titolo:
Prevalence of polymorphisms in the dihydrofolate reductase and dihydropteroate synthetase genes of Plasmodium falciparum isolates from southern Mauritania
Autore:
Eberl, KJ; Jelinek, T; Aida, AO; Peyerl-Hoffmann, G; Heuschkel, C; el Valy, AO; Christophel, EM;
Indirizzi:
Univ Munich, Dept Trop Med & Infect Dis, D-80802 Munich, Germany Univ Munich Munich Germany D-80802 & Infect Dis, D-80802 Munich, Germany Direct Reg Act Sociale & Sante, Hodh El Gharbi, Mauritania Direct Reg Act Sociale & Sante Hodh El Gharbi Mauritania bi, Mauritania Univ Munich, GFA MEDICA, GTZ, Projet Lutte Contre Paludisme Integre Dans Soins, Nonakchott, Mauritania Univ Munich Nonakchott Mauritania re Dans Soins, Nonakchott, Mauritania Projet Sante Base, Hodh El Gharbi, Mauritania Projet Sante Base Hodh El Gharbi Mauritania Hodh El Gharbi, Mauritania
Titolo Testata:
TROPICAL MEDICINE & INTERNATIONAL HEALTH
fascicolo: 10, volume: 6, anno: 2001,
pagine: 756 - 760
SICI:
1360-2276(200110)6:10<756:POPITD>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
THYMIDYLATE SYNTHASE GENE; HUMAN MALARIA PARASITE; CYCLOGUANIL RESISTANCE; SEQUENCE VARIATION; POINT MUTATIONS; PYRIMETHAMINE; SULFADOXINE; PCR;
Keywords:
malaria; Plasmodium falciparum; drug resistance; dihydrofolate reductase; dihydropteroate synthase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Jelinek, T Univ Munich, Dept Trop Med & Infect Dis, Leopoldstr 5, D-80802 Munich, Germany Univ Munich Leopoldstr 5 Munich Germany D-80802 unich, Germany
Citazione:
K.J. Eberl et al., "Prevalence of polymorphisms in the dihydrofolate reductase and dihydropteroate synthetase genes of Plasmodium falciparum isolates from southern Mauritania", TR MED I H, 6(10), 2001, pp. 756-760

Abstract

The increasing resistance of Plasmodium falciparum in the treatment of uncomplicated malaria with pyrimethamine/sulphadoxine has been associated in several studies with the occurrence of point mutations in the genes of dihydrofolate reductase (DHFR) and dihydropteroate synthetase (DHPS). In this study, the prevalence of these mutations was examined in samples from south-east Mauritania, where atypically strong rainfalls in 1998 and 1999 led to asevere outbreak of falciparum malaria. We analysed 386 samples by polymerase chain reaction (PCR) for infection with P. falciparum, of which 162 (41.97%) were positive. These isolates were examined for point mutations in thegenes of DHFR (codons 16, 51, 59, 108 and 164) and DHPS (codons 436, 437, 540, 581 and 613) by nested PCR and subsequent mutation-specific restriction enzyme digest. We found a low overall prevalence of DHFR gene mutations (up to 18.6% of isolates), but a high overall prevalence of DHPS gene mutations (up to 49.1% of isolates). Thus, emerging resistance to antifolate drugs may be expected to develop soon in the investigated area. This study demonstrates the utility of simple, relatively rapid and inexpensive molecular methods and their application in surveillance programmes. Testing for prevalence of point mutations conferring antifolate resistance might help to identify the developing of drug resistance at a very early stage.

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Documento generato il 20/01/20 alle ore 10:44:53