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Titolo:
Mononuclear cells ingest E-coli opsonized by investigational intravenous immunoglobulin preparations in the absence of complement more efficiently than polymorphonuclear phagocytes
Autore:
Tinguely, C; Schaller, M; Nydegger, UE;
Indirizzi:
Univ Hosp Bern, Inselspital, Cardiovasc Surg Clin, CH-3010 Bern, Switzerland Univ Hosp Bern Bern Switzerland CH-3010 Clin, CH-3010 Bern, Switzerland Univ Hosp Bern, Inselspital, Dept Hematol, CH-3010 Bern, Switzerland Univ Hosp Bern Bern Switzerland CH-3010 matol, CH-3010 Bern, Switzerland Scripps Clin & Res Inst, Dept Immunol, La Jolla, CA 92037 USA Scripps Clin& Res Inst La Jolla CA USA 92037 nol, La Jolla, CA 92037 USA
Titolo Testata:
TRANSFUSION AND APHERESIS SCIENCE
fascicolo: 1, volume: 25, anno: 2001,
pagine: 43 - 50
SICI:
1473-0502(200108)25:1<43:MCIEOB>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYVALENT IMMUNOGLOBULINS; IMMUNE GLOBULIN; DRUG-THERAPY; GAMMA; INTERLEUKIN-8; MACROPHAGES; FC; IMMUNODEFICIENCY; ANTIBODIES; RECEPTORS;
Keywords:
phagocytosis; IVIg preparations; Fc gamma Receptor; mononuclear phagocytes; polymorphonuclear leukocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Nydegger, UE Univ Hosp Bern, Inselspital, Cardiovasc Surg Clin, CH-3010 Bern, Switzerland Univ Hosp Bern Bern Switzerland CH-3010 0 Bern, Switzerland
Citazione:
C. Tinguely et al., "Mononuclear cells ingest E-coli opsonized by investigational intravenous immunoglobulin preparations in the absence of complement more efficiently than polymorphonuclear phagocytes", TRANSF AP S, 25(1), 2001, pp. 43-50

Abstract

Objective: The increasing use of intravenous immunoglobulins (IVIg) to treat systemic inflammatory, mostly autoimmune, diseases raises the concern that their competition for Fc gamma Receptor (Fc gamma /R) binding with immune complexes might interfere with the removal of opsonized microbial agents more than it favoured their opsonophagocytosis. Methods: Using a sensitive FACS-based phagocytosis assay allowing for the identification of the phagocytosing cell-type, we have tested the relative capacity of Pentaglobin (R) and two investigational IVIg preparations, IVIgG and IVIgM, to promote E. coli phagocytosis. The plasma IL-8 levels 4 h after phagocytosis were measured by ELISA. Results: Heat inactivated, complement (C) depleted serum allowed about 25%polymorphonuclear leukocytes (PMNLs) and 15% monocytes (MNs) of 16 donors to phagocyte E. coli. The addition of IVIg favoured mean phagocytosis by MNs to 55%, but by PMNLs only to 25%. The addition of fresh autologous plasmaas a C source resulted in a boost of phagocytosing capacity to reach > 90%of the PMNLs and > 72% of the MNs. Blockade of Fc gamma RII and Fc,IRI using monoclonal antibodies (mAb) reduced phagocytosis and IL-S release. Conclusion: We conclude that excess IVIgG/M preparations added to a phagocytic system of PMNLs and MNs, without preopsonization of E. coli, promotes phagocytosis as expected, but MNs rather than PMNLs do this more thoroughlyon their own. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 02:38:05