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Titolo:
Microarray analysis of differential gene expression in lead-exposed astrocytes
Autore:
Bouton, CMLS; Hossain, MA; Frelin, LP; Laterra, J; Pevsner, J;
Indirizzi:
Kennedy Krieger Inst, Dept Neurol, Baltimore, MD 21205 USA Kennedy KriegerInst Baltimore MD USA 21205 urol, Baltimore, MD 21205 USA Johns Hopkins Univ, Dept Neurosci, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 urosci, Baltimore, MD 21205 USA Johns Hopkins Univ, Dept Neurol, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 Neurol, Baltimore, MD 21205 USA Johns Hopkins Univ, Dept Oncol, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 Oncol, Baltimore, MD 21205 USA
Titolo Testata:
TOXICOLOGY AND APPLIED PHARMACOLOGY
fascicolo: 1, volume: 176, anno: 2001,
pagine: 34 - 53
SICI:
0041-008X(20011001)176:1<34:MAODGE>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; ENDOTHELIAL GROWTH-FACTOR; FIBRILLARY ACIDIC PROTEIN; MESSENGER-RNA EXPRESSION; NUCLEAR EXPORT RECEPTOR; BOVINE CHROMAFFIN CELLS; CENTRAL-NERVOUS-SYSTEM; DEVELOPING RAT-BRAIN; AP-1 DNA-BINDING; ANNEXIN-V;
Keywords:
metal; toxicity; microarray; toxicogenomics;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
151
Recensione:
Indirizzi per estratti:
Indirizzo: Pevsner, J Kennedy Krieger Inst, Dept Neurol, 707 N Broadway, Baltimore, MD 21205 USA Kennedy Krieger Inst 707 N Broadway Baltimore MD USA 21205 USA
Citazione:
C.M.L.S. Bouton et al., "Microarray analysis of differential gene expression in lead-exposed astrocytes", TOX APPL PH, 176(1), 2001, pp. 34-53

Abstract

The toxic metal lead is a widespread environmental health hazard that can adversely affect human health. In an effort to better understand the cellular and molecular consequences of lead exposure, we have employed cDNA microarrays to analyze the effects of acute lead exposure on large-scale gene expression patterns in immortalized rat astrocytes. Our studies identified many genes previously reported to be differentially regulated by lead exposure. Additionally, we have identified novel putative targets of lead-mediatedtoxicity, including members of the family of calcium/phospholipid binding annexins, the angiogenesis-inducing thrombospondins, collagens, and tRNA synthetases. We demonstrate the ability to distinguish lead-exposed samples from control or sodium samples solely on the basis of large-scale gene expression patterns using two complementary clustering methods. We have confirmed the altered expression of candidate genes and their encoded proteins by RT-PCR and Western blotting, respectively. Finally, we show that the calcium-dependent phospholipid binding protein annexin A5, initially identified asa differentially regulated gene by our microarray analysis, is directly bound and activated by nanomolar concentrations of lead. We conclude that microarray technology is an effective tool for the identification of lead-induced patterns of gene expression and molecular targets of lead. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:11:56