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Titolo:
Size distribution and meiotic competence of oocytes obtained from bitch ovaries at various stages of the oestrous cycle
Autore:
Otoi, T; Ooka, A; Murakami, M; Karja, NWK; Suzuki, T;
Indirizzi:
Yamaguchi Univ, Dept Vet Sci, Anim Reprod & Biotechnol Lab, Yamaguchi 7538515, Japan Yamaguchi Univ Yamaguchi Japan 7538515 nol Lab, Yamaguchi 7538515, Japan
Titolo Testata:
REPRODUCTION FERTILITY AND DEVELOPMENT
fascicolo: 2-3, volume: 13, anno: 2001,
pagine: 151 - 155
SICI:
1031-3613(2001)13:2-3<151:SDAMCO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATURATION IN-VITRO; DEVELOPMENTAL COMPETENCE; FERTILIZATION; FOLLICLES; DIAMETER; QUALITY; INVITRO;
Keywords:
canine; oocyte diameter;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Otoi, T Yamaguchi Univ, Dept Vet Sci, Anim Reprod & Biotechnol Lab, Yamaguchi 7538515, Japan Yamaguchi Univ Yamaguchi Japan 7538515 Yamaguchi 7538515, Japan
Citazione:
T. Otoi et al., "Size distribution and meiotic competence of oocytes obtained from bitch ovaries at various stages of the oestrous cycle", REPROD FERT, 13(2-3), 2001, pp. 151-155

Abstract

The present study was conducted to examine the effects of the stage of theoestrous cycle on the meiotic competence of canine oocytes and also to investigate the relationship between the stage of the oestrous cycle and the relative size distribution of oocytes obtained from bitches at three stages of the cycle (anoestrus, follicular phase and dioestrus). Only healthy-looking cumulus-oocyte complexes were used for in vitro maturation and these were divided into three groups based on diameter (< 110, 110 to < 120 and greater than or equal to 120 mum). The mean diameter of oocytes from ovaries at anoestrus, the follicular phase and dioestrus was 103.6, 119.2 and 107.7 pm, respectively. The percentage of large oocytes (greater than or equal to120 mum) collected at the follicular phase was higher (P <0.01) than that collected at dioestrus and the percentage of oocytes ! 120 tm collected from ovaries at dioestrus was higher (P <0.01) than that collected at anoestrus. After culture for 72 h, significantly more oocytes reached metaphase II (MII) in the fallicular phase than in the other stages (P <0.01), and more oocytes reached MII in dioestrus than in anoestrus (P <0.05). In the greater than or equal to 120 mum group, the frequency of oocytes that resumed meiosis in the follicular phase was higher (P <0.05) than in the other stages. However, in the smaller diameter (< 120 mum) groups, there were no significant differences between ovaries at different stages of the oestrous cycle with respect to the proportion of oocytes reaching each stage of meiosis. Thus, the oestrous cycle stage influences maturation frequency. Moreover, oocytes demonstrated a size-related ability to undergo meiotic maturation, irrespective of the stage of the oestrous cycle. These results suggest that the effects of the stage of the oestrous cycle may result from differences in the distribution of large oocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 18:37:04