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Titolo:
An in situ hybridization study of the effects of artificial insemination on the localization of cells expressing MHC class II mRNA in the chicken oviduct
Autore:
Zheng, WM; Nishibori, M; Isobe, N; Yoshimura, Y;
Indirizzi:
Hiroshima Univ, Grad Sch Int Dev & Cooperat, Higashihiroshima 7398528, Japan Hiroshima Univ Higashihiroshima Japan 7398528 hihiroshima 7398528, Japan Hiroshima Univ, Fac Appl Biol Sci, Higashihiroshima 7398528, Japan Hiroshima Univ Higashihiroshima Japan 7398528 hihiroshima 7398528, Japan
Titolo Testata:
REPRODUCTION
fascicolo: 4, volume: 122, anno: 2001,
pagine: 581 - 586
SICI:
1470-1626(200110)122:4<581:AISHSO>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR HISTOCOMPATIBILITY COMPLEX; GONADAL-STEROIDS; SPERM STORAGE; TRANSPORT; HEN; AGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Yoshimura, Y Hiroshima Univ, Grad Sch Int Dev & Cooperat, Higashihiroshima7398528, Japan Hiroshima Univ Higashihiroshima Japan 7398528 398528, Japan
Citazione:
W.M. Zheng et al., "An in situ hybridization study of the effects of artificial insemination on the localization of cells expressing MHC class II mRNA in the chicken oviduct", REPRODUCT, 122(4), 2001, pp. 581-586

Abstract

The aim of this study was to determine the effects of artificial insemination on the localization of antigen-presenting cells expressing MHC class IImRNA in chicken oviducts. Laying hens (35 weeks old) were inseminated withfresh semen or sham-inseminated with saline daily for 3 days. in situ hybridization was performed to detect chicken MHC class II (B-LB21 major gene) mRNA on frozen sections of oviductal infundibulum, uterovaginal junction and vagina by using digoxigenin-labelled PCR probes. Cells expressing MHC class II were observed mainly in the oviductal mucosal stroma and occasionallyin the mucosal epithelium. After 24 h, the population of cells expressing MHC class II in the infundibulum was significantly higher in laying hens inseminated with fresh semen than in the control hens sham-inseminated with saline (P < 0.05). However, there was no significant difference in the population of cells expressing MHC class II in the uterovaginal junction and vagina between the artificially inseminated and control hens. These results indicate that anti-sperm immune responses, including the influx of cells expressing MHC class II and enhanced MHC class If mRNA expression, probably occur in the infundibulum after artificial insemination.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 15:42:18