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Titolo:
Dye-affinity hollow-fibres and their lysozyme adsorption-desorption characteristics
Autore:
Senel, S; Akgol, S; Arica, Y; Denizli, A;
Indirizzi:
Univ Hacettepe, Dept Chem, TR-06100 Ankara, Turkey Univ Hacettepe AnkaraTurkey TR-06100 Dept Chem, TR-06100 Ankara, Turkey Kirikkale Univ, Dept Biol, Kirikkale, Turkey Kirikkale Univ Kirikkale Turkey kale Univ, Dept Biol, Kirikkale, Turkey
Titolo Testata:
POLYMER INTERNATIONAL
fascicolo: 10, volume: 50, anno: 2001,
pagine: 1143 - 1149
SICI:
0959-8103(200110)50:10<1143:DHATLA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNOGLOBULIN-G; IMMOBILIZED POLYHYDROXYETHYLMETHACRYLATE MICROBEADS; FIBER MEMBRANES; POLY(2-HYDROXYETHYLMETHACRYLATE) MEMBRANES; TRYPSIN PURIFICATION; PROTEIN ADSORPTION; HUMAN PLASMA; CHROMATOGRAPHY; REMOVAL; SORPTION;
Keywords:
hollow-fibre sorbents; Reactive Green HE-4BD; lysozyme; dye-affinity adsorbents; polyamide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Denizli, A PK 51, TR-06242 Ankara, Turkey PK 51 Ankara Turkey TR-06242PK 51, TR-06242 Ankara, Turkey
Citazione:
S. Senel et al., "Dye-affinity hollow-fibres and their lysozyme adsorption-desorption characteristics", POLYM INT, 50(10), 2001, pp. 1143-1149

Abstract

Dye-affinity adsorption is increasingly used for protein separation. Hollow-fibres have advantages as adsorbents in comparison to conventional bead supports because they are not compressible and can eliminate internal diffusion limitations. The aim of this study was to explore in detail the performance of polyamide hollow-fibres to which Reactive Green HE-4BD was attachedfor adsorption of lysozyme. The hollow-fibre was characterized by scanningelectron microscopy. These dye-carrying hollow-fibres (26.3 mu mol g(-1)) were used in the lysozyme adsorption-elution studies. The effect of initialconcentration of lysozyme and medium pH on the adsorption efficiency of dye-attached hollow-fibres was studied in a batch system. The non-specific adsorption of lysozyme on the polyamide hollow-fibres was 1.8 mgg(-1). Reactive Green HE-4BD attachment significantly increased the lysozyme adsorption up to 41.1 mgg(-1). Langmuir adsorption model was found to be applicable ininterpreting lead adsorption by Reactive Green HE-4BD attached hollow fibres. Significant amount of the adsorbed lysozyme (up to 95%) was eluted in lh in the elution medium containing 1.0M NaSCN at pH 8.0. In order to determine the effects of adsorption conditions on possible conformational changesof lysozyme structure, fluorescence spectrophotometry was employed. We concluded that polyamide dye-affinity hollow-fibres can be applied for lysozyme adsorption without causing any significant conformational changes. Repeated adsorption-elution processes showed that these dye-attached hollow-fibres are suitable for lysozyme adsorption. (C) 2001 Society of Chemical Industry.

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Documento generato il 01/04/20 alle ore 15:38:25