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Titolo:
Role of endogenous nitric oxide generation in the regulation of vascular tone and reactivity in small vessels as investigated in transgenic mice using synchrotron radiation microangiography
Autore:
Yamashita, T; Kawashima, S; Ozaki, M; Namiki, M; Satomi-Kobayashi, S; Seno, T; Matsuda, Y; Inoue, N; Hirata, K; Akita, H; Umetani, K; Tanaka, E; Mori, H; Yokoyama, M;
Indirizzi:
Kobe Univ, Grad Sch Med, Div Cardiovasc & Resp Med, Kobe, Hyogo, Japan Kobe Univ Kobe Hyogo Japan Div Cardiovasc & Resp Med, Kobe, Hyogo, Japan Kobe Univ, Grad Sch Med, Dept Internal Med, Div Gen Med Sci, Kobe, Hyogo, Japan Kobe Univ Kobe Hyogo Japan rnal Med, Div Gen Med Sci, Kobe, Hyogo, Japan Japan Synchrotron Radiat Res Inst, Harima, Japan Japan Synchrotron Radiat Res Inst Harima Japan Res Inst, Harima, Japan Tokai Univ, Sch Med, Dept Physiol, Isehara, Kanagawa, Japan Tokai Univ Isehara Kanagawa Japan Dept Physiol, Isehara, Kanagawa, Japan Tokai Univ, Sch Med, Res Ctr Genet Engn Cell Transplantat, Isehara, Kanagawa, Japan Tokai Univ Isehara Kanagawa Japan Transplantat, Isehara, Kanagawa, Japan
Titolo Testata:
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
fascicolo: 5, volume: 5, anno: 2001,
pagine: 494 - 503
SICI:
1089-8603(200110)5:5<494:ROENOG>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU; SYNTHASE; ARTERIES; VASORELAXATION; VASODILATION;
Keywords:
nitric oxide; endothelial nitric oxide synthase; transgenic mouse; vascular tone; microvascular reactivity; microangiography; synchrotron radiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Kawashima, S Kobe Univ, Grad Sch Med, Div Cardiovasc & Resp Med, Kobe, Hyogo, Japan Kobe Univ Kobe Hyogo Japan c & Resp Med, Kobe, Hyogo, Japan
Citazione:
T. Yamashita et al., "Role of endogenous nitric oxide generation in the regulation of vascular tone and reactivity in small vessels as investigated in transgenic mice using synchrotron radiation microangiography", NITRIC OXID, 5(5), 2001, pp. 494-503

Abstract

Nitric oxide (NO) produced by endothelial NO synthase (eNOS) plays a central role in regulation of vascular tone and reactivity. The purpose of this study is to clarify the basal tone and microvascular reactivity in eNOS-overexpressing transgenic (Tg) mice in vivo with a microangiography system using monochromatic synchrotron radiation (SR). The mouse femoral artery was cannulated, nonionic contrast media was injected, and microangiography was performed in hindlimbs of mice. Serial images of the small blood vessels (diameter < 200 mum) were recorded by the SR microangiography system. At basalconditions, the diameter of tibial arteries in eNOS-Tg mice was larger than that of wild-type mice (179 +/- 8 versus 132 +/- 8 pm; P < 0.01). L-NAME treatment decreased the vessel diameter and canceled the difference in vessel diameters between two genotypes. Acetylcholine- and sodium nitroprusside-induced relaxations of small vessels were significantly reduced in Tg micecompared with wild-type mice (35.0 +/- 9.4 versus 61.6 +/- 6.7%, 85.0 +/- 10.2 versus 97.3 +/- 6.7% of the maximum relaxation, respectively). Our data provide the evidence that overproduced NO from endothelium reduces vascular tone and plays a pivotal role in regulation of vascular tone in small vessels. Furthermore, the reduced NO-mediated relaxation in small vessels of eNOS-Tg mice is demonstrated for the first time in vivo. SR microangiography allows us to evaluate the reactivity in small-sized vessels and appears to be a powerful tool for assessing the microvascular circulation in vivo. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 16:57:39