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Titolo:
Ubiquitin immunoreactivity in presumed spinal interneurones in motor neurone disease
Autore:
Stephens, B; Navarrete, R; Guiloff, RJ;
Indirizzi:
Imerial Coll Sch Med, Dept Neuromuscular Disorders, Div Neurosci & PsycholMed, London, England Imerial Coll Sch Med London England rosci & PsycholMed, London, England
Titolo Testata:
NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY
fascicolo: 5, volume: 27, anno: 2001,
pagine: 352 - 361
SICI:
0305-1846(200110)27:5<352:UIIPSI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMYOTROPHIC-LATERAL-SCLEROSIS; DISMUTASE TRANSGENIC MICE; ANTERIOR HORN CELLS; INTERMEDIATE ZONE; INCLUSIONS; DEGENERATION; PATHOLOGY; CORD; NEUROFILAMENTS; PATHOGENESIS;
Keywords:
amyotrophic lateral sclerosis; anterior horn cell; immunocytochemistry; interneurone; motor neurone; motor neurone disease; pathology; ubiquitin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Guiloff, RJ Charing Cross Hosp, W London Neurosci Ctr, Neuromuscular Unit,London W6 8RF, England Charing Cross Hosp London England W6 8RF don W6 8RF, England
Citazione:
B. Stephens et al., "Ubiquitin immunoreactivity in presumed spinal interneurones in motor neurone disease", NEUROP AP N, 27(5), 2001, pp. 352-361

Abstract

Previous studies have demonstrated the presence of ubiquitin-immunoreactivity (Ub-IR) as inclusions and skeins in motor neurones of both the familialand sporadic forms of motor neurone disease (MND). There is evidence that interneurones also degenerate in MND, but Ub-IR in ventral horn spinal interneurones has not been studied previously. Here, Ub-IR was investigated in 1445 presumed interneurones and 1086 presumed motor neurones counted in three random 20-mum sections of the ventral horn of the third lumbar segment of the spinal cord of each of seven controls and seven patients with MND. The ventral horn was divided into four quadrants; the dorsomedial quadrant contains almost exclusively interneurones and the ventrolateral quadrant largely motor neurones. The neurones were also classified by morphological and size criteria into presumed interneurones (<25 <mu>m) and presumed motor neurones (greater than or equal to 25 mum). Ub-IR was classified as inclusions, skeins and dispersed cytoplasmic and nuclear staining. Ub-IR inclusions or skeins were not observed in the controls but 6.6% of neurones (motor neurones and interneurones) showed the presence of dispersed cytoplasm staining and nuclear staining. The incidence of Ub-IR cytoplasmic and nuclear staining was significantly greater in both motor neurones and interneurones of MND patients than controls. Ub-IR was less frequent in MND cases in which agreat loss of neurones was observed. Ub-IR was significantly more frequentin motor neurones than interneurones, both in patients and controls. Ub-IRinclusions and skeins were only observed in motor neurones from MND patients. Ub-IR inclusions were not observed in presumed spinal interneurones, while skeins were only seen in three out of 565 of these cells (two of them in the dorsomedial quadrant) in two out of seven patients. Thus, although presumed spinal interneurones occasionally revealed Ub-IR features similar tomotor neurones, the rare staining of Ub-IR skeins and the lack of Ub-IR inclusions in interneurones in MND suggests that these neurones only occasionally form ubiquitin-protein conjugates. Neuronal size, rather than type, may be important in determining whether ubiquitin-protein conjugates form in the ventral horn neurones in MND.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 02:47:58