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Titolo:
Free cytosolic Ca2+ recordings from myenteric neurones in multilayer intestinal preparations
Autore:
Vanden Berghe, P; Missiaen, L; Bellon, E; Vanderwinden, JM; Janssens, J; Tack, J;
Indirizzi:
Katholieke Univ Leuven, Ctr Gastroenterol Res, B-3000 Louvain, Belgium Katholieke Univ Leuven Louvain Belgium B-3000 s, B-3000 Louvain, Belgium Katholieke Univ Leuven, Physiol Lab, B-3000 Louvain, Belgium Katholieke Univ Leuven Louvain Belgium B-3000 b, B-3000 Louvain, Belgium Katholieke Univ Leuven, Med Image Comp ESAT & Radiol, B-3000 Louvain, Belgium Katholieke Univ Leuven Louvain Belgium B-3000 l, B-3000 Louvain, Belgium Free Univ Brussels, Neurophysiol Lab, Brussels, Belgium Free Univ Brussels Brussels Belgium Neurophysiol Lab, Brussels, Belgium
Titolo Testata:
NEUROGASTROENTEROLOGY AND MOTILITY
fascicolo: 5, volume: 13, anno: 2001,
pagine: 493 - 502
SICI:
1350-1925(200110)13:5<493:FCCRFM>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PIG SMALL-INTESTINE; ENTERIC NERVOUS-SYSTEM; SYNAPTIC TRANSMISSION; ELECTRICAL-ACTIVITY; CIRCULAR MUSCLE; MOTOR-NEURONS; PLEXUS; ILEUM; CELLS; EXCITABILITY;
Keywords:
confocal microscopy; DiI; ICC; Indo-1; myenteric plexus; optical;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Tack, J Katholieke Univ Leuven, Ctr Gastroenterol Res, Herestr 49, B-3000 Louvain,Belgium Katholieke Univ Leuven Herestr 49 Louvain Belgium B-3000 ,Belgium
Citazione:
P. Vanden Berghe et al., "Free cytosolic Ca2+ recordings from myenteric neurones in multilayer intestinal preparations", NEUROG MOT, 13(5), 2001, pp. 493-502

Abstract

The ability to simultaneously monitor different myenteric neurones in a multilayer preparation may enhance our understanding of the enteric nervous system. Longitudinal muscle myenteric plexus prepar- ations were mounted in recording chambers with a coverslip base and loaded with Indo-1-AM. cytosolic Ca2+ concentration ([Ca2+](i)); changes were recorded at room temperature with a confocal microscope. In addition to mechanical (pressure-ring) andpharmacological (nifedipine) reduction of muscle contractions, purpose-designed software was developed to reposition regions of interest and avoid artefacts. Confocal scanning permitted optical selection of single cell layers. High K+ depolarization, used to distinguish between excitable and nonexcitable cells, caused a synchronous [Ca2+](i) rise in 84.3% of the ganglion cells. Acetylcholine, substance P and serotonin (all at 10(-5)mol L-1) induced transient [Ca2+](i) changes in subpopulations of myenteric neurones (45.1%, 42.9 and 21.9%, respectively). In addition to immediate responses to agonists, delayed [Ca2+](i) changes were also recorded, suggesting the presence of both directly activated and synaptically driven neurones. Functionally identified neurones and other cells in close apposition to the ganglia (interstitial cells of Cajal) could also be studied. This study demonstratesthe potential of optical Ca2+ recordings to monitor spread of activity in myenteric neurones and to study their interaction with non-neuronal targets.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:09:52