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Titolo:
Intra-abdominal injection of double-stranded RNA into anesthetized adult Drosophila triggers RNA interference in the central nervous system
Autore:
Dzitoyeva, S; Dimitrijevic, N; Manev, H;
Indirizzi:
Univ Illinois, Inst Psychiat, Dept Psychiat, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 , Dept Psychiat, Chicago, IL 60612 USA
Titolo Testata:
MOLECULAR PSYCHIATRY
fascicolo: 6, volume: 6, anno: 2001,
pagine: 665 - 670
SICI:
1359-4184(200111)6:6<665:IIODRI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUNCTIONAL GENOMIC ANALYSIS; MESSENGER-RNA; GENE; ELEGANS; DEGRADATION;
Keywords:
RNAi; gene silencing; fruit fly; CNS; lacZ; beta galactosidase; fluoxetine; Nrf; antidepressant;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Manev, H Univ Illinois, Inst Psychiat, Dept Psychiat, 1601 W Taylor St,MC912, Chicago, IL 60612 USA Univ Illinois 1601 W Taylor St,MC912 Chicago IL USA 60612 612 USA
Citazione:
S. Dzitoyeva et al., "Intra-abdominal injection of double-stranded RNA into anesthetized adult Drosophila triggers RNA interference in the central nervous system", MOL PSYCHI, 6(6), 2001, pp. 665-670

Abstract

RNA interference (RNAi) is a gene silencing mechanism that can be triggered by introducing double-stranded RNA (dsRNA) into cells expressing the appropriate molecular machinery, which then degrades the corresponding endogenous mRNA. RNA! can be used for determining gene function and creating functional 'knockout' organisms. Here we show for the first time that RNAi can beinduced in adult fruit files by injecting dsRNA into the abdomen of anesthetized Drosophila, and that this method can also target genes expressed in the central nervous system (CNS). Two genes were targeted to investigate the effects of dsRNA injection on their mRNA content; lacZ transgene (expressed either in the gut or in the CNS), and GM06434, the Drosophila homologue of the C. elegans gene nrf (nose resistant to fluoxetine). Both the transgene and the endogenous gene were successfully silenced in adult Drosophila by intra-abdominal injection of their respective dsRNA. We propose that our method of RNAi in adult flies can be used to characterize gene functioning in the CNS without the typical interference in development found in most gene mutation studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:02:46