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Titolo:
Structure, organization and characterization of the gene cluster involved in the production of microcin E492, a channel-forming bacteriocin
Autore:
Lagos, R; Baeza, M; Corsini, G; Hetz, C; Strahsburger, E; Castillo, JA; Vergara, C; Monsterio, O;
Indirizzi:
Univ Chile, Fac Ciencias, Dept Biol, Santiago, Chile Univ Chile SantiagoChile ile, Fac Ciencias, Dept Biol, Santiago, Chile
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 1, volume: 42, anno: 2001,
pagine: 229 - 243
SICI:
0950-382X(200110)42:1<229:SOACOT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; KLEBSIELLA-PNEUMONIAE; ABC TRANSPORTERS; PROTEIN; IDENTIFICATION; EXPORT; HEMOLYSIN; SECRETION; IMMUNITY; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Lagos, R Univ Chile, Fac Ciencias, Dept Biol, Casilla 653, Santiago, ChileUniv Chile Casilla 653 Santiago Chile illa 653, Santiago, Chile
Citazione:
R. Lagos et al., "Structure, organization and characterization of the gene cluster involved in the production of microcin E492, a channel-forming bacteriocin", MOL MICROB, 42(1), 2001, pp. 229-243

Abstract

Microcin E492 is a low-molecular-weight, channel-forming bacteriocin produced and excreted by Klebsiella pneumoniae RYC492. A 13 kb chromosomal DNA fragment from K. pneumoniae RYC492 was sequenced, and it was demonstrated byrandom Tn5 mutagenesis that most of this segment, which has at least 10 cistrons, is needed for the production of active microcin and its immunity protein. Genes mceG and mceH correspond to an ABC exporter and its accessory protein, respectively, and they are closely related to the colicin V ABC export system. The microcin E492 system also requires the product of gene mceF as an additional factor for export. Despite the fact that this bacteriocin lacks post-translational modifications, genes mceC, mcel and mceJ are needed for the production of active microcin. Genes mceC and mcel are homologous to a glycosyl transferase and acyltransferase, respectively, whereas mceJ has no known homologue. Mutants in these three genes secrete an inactive form of microcin, able to form ion channels in a phospholipidic bilayer, indicating that the mutation of these microcin genes does not alter the process of membrane insertion. On the other hand, microcin isolated from mutantsin genes mceC and mceJ has a lethal effect when incubated with spheroplasts of sensitive cells, indicating that the microcin defects in these mutantsare likely to alter receptor recognition at the outer membrane. A model for synthesis and export is proposed as well as a novel maturation pathway that would involve conformational changes to explain the production of activemicrocin E492.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 14:29:30