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Titolo:
The regulation and localization of angiopoietin-1,-2, and their receptor Tie2 in normal and pathologic human placentae
Autore:
Zhang, EG; Smith, SK; Baker, PN; Charnock-Jones, DS;
Indirizzi:
Univ Cambridge, Rosie Hosp, Dept Obstet & Gynaecol, Reprod Mol Res Grp, Cambridge CB2 2SW, England Univ Cambridge Cambridge England CB2 2SW Grp, Cambridge CB2 2SW, England Univ Nottingham, City Hosp, Sch Human Dev, Nottingham NG5 1PB, England Univ Nottingham Nottingham England NG5 1PB , Nottingham NG5 1PB, England
Titolo Testata:
MOLECULAR MEDICINE
fascicolo: 9, volume: 7, anno: 2001,
pagine: 624 - 635
SICI:
1076-1551(200109)7:9<624:TRALOA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; VASCULAR-PERMEABILITY FACTOR; BLOOD-VESSEL FORMATION; MESSENGER-RNA LEVELS; TYROSINE KINASE; GESTATIONAL-AGE; CHORIONIC-GONADOTROPIN; MATERNAL CIRCULATION; PRE-ECLAMPSIA; CELL GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Charnock-Jones, DS Univ Cambridge, Rosie Hosp, Dept Obstet & Gynaecol, Reprod Mol Res Grp, Cambridge CB2 2SW, England Univ Cambridge Cambridge England CB2 2SW 2SW, England
Citazione:
E.G. Zhang et al., "The regulation and localization of angiopoietin-1,-2, and their receptor Tie2 in normal and pathologic human placentae", MOL MED, 7(9), 2001, pp. 624-635

Abstract

Background: Angiopoietin-1 (Ang-1) and its antagonist angiopoietin-2 (Ang-2) act on the endothelial cell Tie-2 receptor to regulate vascular integrity and remodeling. The local balance of these factors and the level of otherangiogenic factors determine whether blood vessels grow, are maintained orregress. Profound angiogenesis and vascular remodeling occur in the placenta and this is altered in preeclampsia, a major cause of maternal and fetalmorbidity and mortality. Materials and Methods: The mRNAs encoding Ang-1, Ang-2 and Tie-2 were detected and localized in human placentae throughout gestation. The mechanism of regulation angiopoietin mRNAs level was determined by explant culture in ambient and reduced oxygen, and in the presence of actinomycin D. Results: In situ hybridization showed that Ang-2 mRNA was abundant in the syncytiotrophoblast in the first trimester of human pregnancy. Ang-1 mRNA could not be detected by in situ hybridization, but was by reverse transcriptase-polymerase chain reaction (RT-PCR) and Northern blotting. Placental vascular structure is altered in preeclampsia and intrauterine growth restriction, conditions where feto-placental oxygenation is perturbed. In villous explant cultures, a reduction in oxygen tension significantly raised the levels of Ang-2 mRNA, and this was dependent on transcription. However, similar experiments showed that the stability of the Ang-1 message was greatly reduced under these conditions. Thus, hypoxia has opposite effects on Ang-1 and Ang-2 mRNA levels. Placentae obtained from women with preeclampsia had reduced levels of Ang-2 mRNA compared to gestationally matched controls. There was no difference in the levels of Ang-1 mRNAs. Conclusions: These data show that the relative levels of Ang-1 and Ang-2 mRNA are regulated by local oxygen tension by different mechanisms and that this may be important during normal human placentation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:49:32