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Titolo:
Epididymal epithelium immortalized by simian virus 40 large T antigen: a model to study epididymal gene expression
Autore:
Telgmann, R; Brosens, JJ; Kappler-Hanno, K; Ivell, R; Kirchhoff, C;
Indirizzi:
Univ Hamburg, IHF Inst Hormon & Fortpflanzungsforsch, D-22529 Hamburg, Germany Univ Hamburg Hamburg Germany D-22529 ngsforsch, D-22529 Hamburg, Germany Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Dept ReprodSci & Med, London, England Univ London Imperial Coll Sci Technol & Med London England don, England
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 10, volume: 7, anno: 2001,
pagine: 935 - 945
SICI:
1360-9947(200110)7:10<935:EEIBSV>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYOMAVIRUS ENHANCER ACTIVATOR-3; LEUKOCYTE PROTEASE INHIBITOR; MESSENGER-RIBONUCLEIC-ACID; ESTROGEN-RECEPTOR-ALPHA; MAJOR SECRETORY PROTEIN; ETS ONCOGENE FAMILY; DOG EPIDIDYMIS; EFFERENT DUCTULES; MOLECULAR-CLONING; RAT EPIDIDYMIS;
Keywords:
epididymis; HE1; HE4; immortalization; transcription factors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Kirchhoff, C Univ Hamburg, IHF Inst Hormon & Fortpflanzungsforsch, Grandweg 64, D-22529Hamburg, Germany Univ Hamburg Grandweg 64 Hamburg Germany D-22529 rg, Germany
Citazione:
R. Telgmann et al., "Epididymal epithelium immortalized by simian virus 40 large T antigen: a model to study epididymal gene expression", MOL HUM REP, 7(10), 2001, pp. 935-945

Abstract

Primary cultures of the differentiated, adult epididymal duct epithelium were immortalized by retroviral transduction with the simian virus (SV)40 large T antigen. The canine epididymis was chosen here as a model with high human relevance, representing a convenient and acceptable source of differentiated epididymal tissue and, compared to other animal models, expressing arelatively large number of gene products which are also expressed by the human epididymis. To determine whether the immortalized canine epididymal (IMCE) cells retained a phenotype comparable to the original tissue, epithelial cytokeratins, various epididymal transcription factors as well as mRNAs encoding abundant epididymal secretory proteins, were studied as molecular markers. All IMCE populations obtained after transduction were of epithelial origin. The nuclear androgen receptor (AR) and the polyoma enhancer activator (PEA3), as well as the epididymal mRNA encoding the canine counterparts of human HE1, HE4 and HE5/CD52 epididymal mRNA, were retained in all populations tested. The majority of tested clones were oestrogen receptor ER alpha -positive, but ER beta -negative, while one ER alpha -negative cell population was positive for ER beta. The IMCE populations described thus represent useful permanent tools for studying gene expression of the epididymal duct epithelium, and for other types of experiments, examples including drug effects and toxicity on the epididymis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 07:29:20