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Titolo:
Expression profiles of COUP-TF, DAX-1, and SF-1 in the human adrenal glandand adrenocortical tumors: Possible implications in steroidogenesis
Autore:
Shibata, H; Ikeda, Y; Mukai, T; Morohashi, K; Kurihara, I; Ando, T; Suzuki, T; Kobayashi, S; Murai, M; Saito, I; Saruta, T;
Indirizzi:
Keio Univ, Sch Med, Dept Internal Med, Shinjuku Ku, Tokyo 1608582, Japan Keio Univ Tokyo Japan 1608582 nal Med, Shinjuku Ku, Tokyo 1608582, Japan Keio Univ, Sch Med, Ctr Hlth, Tokyo 1608582, Japan Keio Univ Tokyo Japan1608582 v, Sch Med, Ctr Hlth, Tokyo 1608582, Japan Univ Tsukuba, Inst Basic Med Sci, Dept Anat, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 sukuba, Ibaraki 3058575, Japan Japan Sci & Technol, Core Res Evolutional Sci & Technol, Natl Inst Basic Biol, Dept Dev Biol, Okazaki, Aichi 4448585, Japan Japan Sci & Technol Okazaki Aichi Japan 4448585 aki, Aichi 4448585, Japan Kyushu Univ, Grad Sch Med Sci, Dept Med & Bioregulatory Sci, Fukuoka, Japan Kyushu Univ Fukuoka Japan Dept Med & Bioregulatory Sci, Fukuoka, Japan
Titolo Testata:
MOLECULAR GENETICS AND METABOLISM
fascicolo: 1-2, volume: 74, anno: 2001,
pagine: 206 - 216
SICI:
1096-7192(200109/10)74:1-2<206:EPOCDA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROMOTER-TRANSCRIPTION FACTOR; HORMONE-RECEPTOR SUPERFAMILY; BOVINE CYP17 GENE; COREPRESSOR N-COR; FACTOR-I; ENDOCRINE DEVELOPMENT; PRIMARY ALDOSTERONISM; HYPOPLASIA CONGENITA; 17,20-LYASE ACTIVITY; THERAPEUTIC TARGET;
Keywords:
COUP-TF; DAX-1; SF-1; CYP17; steroidogenesis; adrenal tumor; Cushing syndrome; deoxycorticosterone-producing adenomas;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Shibata, H Keio Univ, Sch Med, Dept Internal Med, Shinjuku Ku, 35 Shinaromachi, Tokyo1608582, Japan Keio Univ 35 Shinaromachi Tokyo Japan 1608582 yo1608582, Japan
Citazione:
H. Shibata et al., "Expression profiles of COUP-TF, DAX-1, and SF-1 in the human adrenal glandand adrenocortical tumors: Possible implications in steroidogenesis", MOL GEN MET, 74(1-2), 2001, pp. 206-216

Abstract

Chicken ovalbumin upstream promoter-transcription factor (COUP-TF), DAX-1,and steroidogenic factor-1 (SF-1) are orphan members of the nuclear hormone receptor superfamily. COUP-TF and DAX-1 have been shown to negatively regulate the transcriptional activity of SF-1, a steroidogenic cell-specific activator of various steroidogenic cytochrome P450 genes. We therefore examined the expression levels and immunolocalization of COUP-TF, DAX-1, and SF-1 in human adrenal gland (NL) and adrenocortical adenomas, and compared theresults with CYP17 expression levels and its enzyme activities to study their potential correlation with adrenocortical steroidogenesis. In NL (n = 10), expressions of COUP-TF, DAX-1, and SF-1 were detected in the nuclei of adrenocortical cells, but not in the medulla. In cortisol-producing adenomas causing Cushing syndrome (CS, n = 20), CYP17 expression was upregulated (298 +/- 2% vs NL 98 +/- 4%), whereas expression levels of both COUP-TFs (COUP-TFI, 52 +/- 5% vs NL 98 +/- 4%; COUP-TFII, 18 +/- 4% vs NL 98 +/- 4%) and DAX-1 (42 +/- 4% vs NL 100 +/- 4%) were reduced. In deoxycorticosterone-producing adenomas (DOC, n = 2), on the other hand, CYP17 expression was extremely reduced (8 and 12% vs NL 98 +/- 4%), whereas DAX-1 expression increased markedly (350 and 360% vs NL 100 +/- 4%). Expression levels of SF-1 didnot differ between NL (100 +/- 8%) and CS (106 +/- 10%), but its expression appeared to be decreased in DOC (25 and 20%). These results showed CYP17 expression to be upregulated and downregulated in CS and DOC, respectively,in a manner reciprocal to that of its repressors, COUP-TF and/or DAX-1. Insummary, the results indicate that co-localization. of COUP-TF, DAX-1, andSF-1 in NL was lost in adrenocortical tumors and that these orphan receptors play an important role in the regulation of steroidogenesis in human adrenals. (C) 2001 Academic Press.

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Documento generato il 23/01/20 alle ore 03:34:29