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Titolo:
Rab3A is involved in transport of synaptic vesicles to the active zone in mouse brain nerve terminals
Autore:
Leenders, AGM; da Silva, FHL; Ghijsen, WEJM; Verhage, M;
Indirizzi:
Univ Utrecht, Med Ctr, Rudolf Magnus Inst, NL-3584 CG Utrecht, NetherlandsUniv Utrecht Utrecht Netherlands NL-3584 CG 3584 CG Utrecht, Netherlands Univ Amsterdam, Inst Neurobiol, Grad Sch Neurosci, NL-1098 SM Amsterdam, Netherlands Univ Amsterdam Amsterdam Netherlands NL-1098 SM M Amsterdam, Netherlands
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 10, volume: 12, anno: 2001,
pagine: 3095 - 3102
SICI:
1059-1524(200110)12:10<3095:RIIITO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
READILY RELEASABLE POOL; NEUROTRANSMITTER RELEASE; MEMBRANE-FUSION; EXOCYTOSIS; PROTEIN; SYNAPSES; RABPHILIN-3A; SECRETION; BINDING; GTPASES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Verhage, M Free Univ Amsterdam, Dept Neurogenom, Boelelaan 1087, NL-1081 HV Amsterdam, Netherlands Free Univ Amsterdam Boelelaan 1087 Amsterdam Netherlands NL-1081 HV
Citazione:
A.G.M. Leenders et al., "Rab3A is involved in transport of synaptic vesicles to the active zone in mouse brain nerve terminals", MOL BIOL CE, 12(10), 2001, pp. 3095-3102

Abstract

The rab family of GTP-binding proteins regulates membrane transport between intracellular compartments. The major rab protein in brain, rab3A, associates with synaptic vesicles. However, rab3A was shown to regulate the fusion probability of synaptic vesicles, rather than their transport and docking. We tested whether rab3A has a transport function by analyzing synaptic vesicle distribution and exocytosis in rab3A null-mutant mice. Rab3A deletiondid not affect the number of vesicles and their distribution in resting nerve terminals. The secretion response upon a single depolarization was alsounaffected. In normal mice, a depolarization pulse in the presence of Ca2induces an accumulation of vesicles close to and docked at the active zone(recruitment). Rab3A deletion completely abolished this activity-dependentrecruitment, without affecting the total number of vesicles. Concomitantly, the secretion response in the rab3A-deficient terminals recovered slowly and incompletely after exhaustive stimulation, and the replenishment of docked vesicles after exhaustive stimulation was also impaired in the absence of rab3A. These data indicate that rab3A has a function upstream of vesiclefusion in the activity-dependent transport of synaptic vesicles to and their docking at the active zone.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 22:40:47